Characterization of the extracellular matrix during somitogenesis in the long-tailed monkey (Macaca fascicularis)

P. E. Peterson, C. S.T. Pow, D. B. Wilson, Andrew G Hendrickx

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The composition of the extracellular matrix (ECM) associated with somitogenesis (stages 10-14) in the long-tailed monkey (Macaca fascicularis) was examined using peroxidase immunocytochemistry. A distinct temporal and spatial staining pattern was demonstrated for each component examined. Fibronectin (FN), laminin (LM) and hyaluronic acid (HA) were associated with the somite at each developmental stage, while no chondroitin sulfate (CS) staining was evident until stages 13/14. The basement membrane and matrix fibers linking the somites to the surrounding epithelia stained for FN, LM and HA. Localization of LM and HA between somite cells was observed at each stage, whereas FN and CS reactivity within the somitic tissue was only observed between sclerotome cells at stages 13/14. Whereas FN- and HA-positive fibers surrounded the unsegmented mesoderm, HA, and to a lesser extent LM, were located within the presomitic tissue. Comparisons are made with earlier studies conducted in rodent and avian embryos which indicate that these ECM components may play a regulatory role in primate somitogenesis.

Original languageEnglish (US)
Pages (from-to)223-233
Number of pages11
JournalActa Anatomica
Volume146
Issue number4
StatePublished - 1993
Externally publishedYes

Keywords

  • chondroitin sulfate
  • fibronectin
  • hyaluronic acid
  • immunocytochemistry
  • laminin
  • long-tailed monkey
  • somites

ASJC Scopus subject areas

  • Anatomy

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  • Cite this

    Peterson, P. E., Pow, C. S. T., Wilson, D. B., & Hendrickx, A. G. (1993). Characterization of the extracellular matrix during somitogenesis in the long-tailed monkey (Macaca fascicularis). Acta Anatomica, 146(4), 223-233.