Characterization of the diffuse mucosal associated lymphoid tissue of feline small intestine

P. Roccabianca, J. C. Woo, Peter F Moore

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Characterization of the feline intestinal mucosal associated lymphoid tissue (MALT) will facilitate investigation of intestinal disease in the cat and promote the cat as an animal model for a range of human diseases which involve the intestinal lymphoid tissue. This includes inflammatory bowel disease, viral and non-viral associated intestinal lymphomas and immunodeficiency associated syndromes. Morphologic and phenotypic characterization of the normal small intestinal diffuse MALT in 22 SPF cats was performed using flow cytometry and cytology on isolated intestinal leukocytes from the intra-epithelial and lamina proprial compartments, as well as immunohistology on tissues from the feline duodenum, jejunum and ileum. The intra-epithelial compartment (IEC) was dominated by lymphocytes (>85%) which frequently contained intracytoplasmic granules. The most striking findings in the IEC were the elevated percentages of CD8α+ lymphocytes (40%), presumed to express CD8αα chains, and CD4-/CD8- (double negative) lymphocytes (44%), and the consistent presence of a minor subpopulation of CD3+/CD11d+ IELs (6%). Small percentages of CD4+ lymphocytes (10%) were observed such that the IEL CD4:CD8 ratio (0.25) was low. The LPC also contained a majority of T cells and few plasma cells. However, this compartment had reduced percentages of CD8α+ lymphocytes (28%) and increased percentages of CD4+ lymphocytes (27%) relative to the IEC. However, the LPL CD4:CD8 ratio (1.0) remained low compared with the ratio in peripheral blood. In feline MALT, MHC class II expression was lower than in other peripheral lymphoid compartments. The results of this study provide important reference values for future investigations involving feline intestinal lymphocytes and demonstrates that the leukocyte distribution and phenotypic characteristics of the feline diffuse MALT appear largely similar to the murine, rat and human counterparts. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)27-42
Number of pages16
JournalVeterinary Immunology and Immunopathology
Volume75
Issue number1-2
DOIs
StatePublished - Jun 30 2000

Fingerprint

Felidae
Lymphoid Tissue
Small Intestine
small intestine
Lymphocytes
cats
lymphocytes
CD4-CD8 Ratio
Cats
Leukocytes
leukocytes
Intestinal Diseases
Jejunum
Plasma Cells
tissues
inflammatory bowel disease
Ileum
Inflammatory Bowel Diseases
Duodenum
plasma cells

Keywords

  • Feline
  • Lymphocyte
  • MALT
  • Mucosal
  • Phenotype
  • Small intestine

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Immunology
  • veterinary(all)

Cite this

Characterization of the diffuse mucosal associated lymphoid tissue of feline small intestine. / Roccabianca, P.; Woo, J. C.; Moore, Peter F.

In: Veterinary Immunology and Immunopathology, Vol. 75, No. 1-2, 30.06.2000, p. 27-42.

Research output: Contribution to journalArticle

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