Characterization of the antigen (gp600) of Heymann nephritis

Sudesh P Makker, A. K. Singh

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

The nephritogenic brush border glycoprotein antigen (gp600) of autoimmune membranous glomerulonephropathy (Heymann nephritis) isolated from crude proximal renal tubular membrane fraction (FX1A) by Lens culinaris lectin affinity chromatography was characterized. The molecular weight of the antigen determined by molecular exclusion chromatography was found to be 600,000. On sodium dodecyl sulfate-polyacrylamide gel electrophoresis it resolved into five polypeptides which were in the molecular weight range of 70,000 to 330,000. All five polypeptide bands stained for carbohydrates and lipids with Schiff's reagent and Sudan black, respectively. Rabbits immunized with gp600 produced an antibody that specifically reacted with the brush border of proximal renal tubules of rat by indirect immunofluorescence and gave a single precipitin line in double diffusion in gel (Ouchterlony) against the antigen. The specificity of gp600 was proven by (a) the ability of gp600 to produce active Heymann nephritis, (b) the ability of anti-gp600 to produce passive Heymann nephritis, and (c) the ability of gp600 alone, out of other antigens in FX1A, to specifically react with the nephritogenic autoantibody. The latter was tested by an enzyme immunodiffusion technique described in this report.

Original languageEnglish (US)
Pages (from-to)287-293
Number of pages7
JournalLaboratory Investigation
Volume50
Issue number3
StatePublished - 1984
Externally publishedYes

Fingerprint

Membranous Glomerulonephritis
Antigens
Microvilli
Molecular Weight
Precipitins
Proximal Kidney Tubule
Peptides
Sudan
Immunodiffusion
Indirect Fluorescent Antibody Technique
Affinity Chromatography
Sodium Dodecyl Sulfate
Autoantibodies
Gel Chromatography
Polyacrylamide Gel Electrophoresis
Glycoproteins
Gels
Carbohydrates
Rabbits
Kidney

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Characterization of the antigen (gp600) of Heymann nephritis. / Makker, Sudesh P; Singh, A. K.

In: Laboratory Investigation, Vol. 50, No. 3, 1984, p. 287-293.

Research output: Contribution to journalArticle

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