Abstract
The selcriiviiy of Lym-1 for malignant li-jympliocytns makes this mono clonal antibody a promising randidaic for tho d('li\''i\ <>| toxit agents îo nia lignant It-cells. The original imrnunogen ued for the devflopmrnl of l.ym 1 was Raji Hnrkitt's lyniphoma cell nuclei [Kpstein AI,. Marder RJ. Winter -IX. Stathopouluh K. ('hen FM. Parker .IW. 'ray'or ('H I 17) Cancer Res -17:S:j()]. The Lym-1 antigen was characterised at that lime ,is a polymorphic HLA-DR variant. UV prepared an affinity column using immnhili/,ed I.yni-1 tn isolate t lie Lym-1 antigen from Raji cell lysate. Iiiniiunologi( al characierizalion of t he immnnoafïinity [mrified Lym-1 antigen on western blots led to the conclusion t hat the antigen i- thebeta chain of ULA-DRK). This was coitfinned by Ld man sequeiK'ine, oi the isolated polypeptide chain. V\estern blot.s fnrtiier stiuu that the Lym-1 epitope is only rerognued if the beta chain disnlfulr bonds are intact. These results imply that Lym 1 binds a discontinuous' epiîope on the beta chain of HLA-DR1Ü.
| Original language | English (US) |
|---|---|
| Journal | FASEB Journal |
| Volume | 11 |
| Issue number | 9 |
| State | Published - 1997 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Cell Biology
Cite this
Characterization of the ant ige nie epitope recognized by tumor selective antibody. / Rose, I. M.; Denardo, D. L.; Meares, C. F.
In: FASEB Journal, Vol. 11, No. 9, 1997.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization of the ant ige nie epitope recognized by tumor selective antibody
AU - Rose, I. M.
AU - Denardo, D. L.
AU - Meares, C. F.
PY - 1997
Y1 - 1997
N2 - The selcriiviiy of Lym-1 for malignant li-jympliocytns makes this mono clonal antibody a promising randidaic for tho d('li\''i\ <>| toxit agents îo nia lignant It-cells. The original imrnunogen ued for the devflopmrnl of l.ym 1 was Raji Hnrkitt's lyniphoma cell nuclei [Kpstein AI,. Marder RJ. Winter -IX. Stathopouluh K. ('hen FM. Parker .IW. 'ray'or ('H I 17) Cancer Res -17:S:j()]. The Lym-1 antigen was characterised at that lime ,is a polymorphic HLA-DR variant. UV prepared an affinity column using immnhili/,ed I.yni-1 tn isolate t lie Lym-1 antigen from Raji cell lysate. Iiiniiunologi( al characierizalion of t he immnnoafïinity [mrified Lym-1 antigen on western blots led to the conclusion t hat the antigen i- thebeta chain of ULA-DRK). This was coitfinned by Ld man sequeiK'ine, oi the isolated polypeptide chain. V\estern blot.s fnrtiier stiuu that the Lym-1 epitope is only rerognued if the beta chain disnlfulr bonds are intact. These results imply that Lym 1 binds a discontinuous' epiîope on the beta chain of HLA-DR1Ü.
AB - The selcriiviiy of Lym-1 for malignant li-jympliocytns makes this mono clonal antibody a promising randidaic for tho d('li\''i\ <>| toxit agents îo nia lignant It-cells. The original imrnunogen ued for the devflopmrnl of l.ym 1 was Raji Hnrkitt's lyniphoma cell nuclei [Kpstein AI,. Marder RJ. Winter -IX. Stathopouluh K. ('hen FM. Parker .IW. 'ray'or ('H I 17) Cancer Res -17:S:j()]. The Lym-1 antigen was characterised at that lime ,is a polymorphic HLA-DR variant. UV prepared an affinity column using immnhili/,ed I.yni-1 tn isolate t lie Lym-1 antigen from Raji cell lysate. Iiiniiunologi( al characierizalion of t he immnnoafïinity [mrified Lym-1 antigen on western blots led to the conclusion t hat the antigen i- thebeta chain of ULA-DRK). This was coitfinned by Ld man sequeiK'ine, oi the isolated polypeptide chain. V\estern blot.s fnrtiier stiuu that the Lym-1 epitope is only rerognued if the beta chain disnlfulr bonds are intact. These results imply that Lym 1 binds a discontinuous' epiîope on the beta chain of HLA-DR1Ü.
UR - http://www.scopus.com/inward/record.url?scp=33750252507&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33750252507&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:33750252507
VL - 11
JO - FASEB Journal
JF - FASEB Journal
SN - 0892-6638
IS - 9
ER -