Abstract
A genomic region encompassing 800 bp of the promoter-regulatory region and exon 1 of the gene (LGP) encoding rat liver glycogen phosphorylase has been isolated and characterized. Transcripts of the LGP gene initiate predominantly within an 8-bp region 48-bp upstream from the start codon. Additional transcripts were detected that initiate as far as 95 bp upstream from the start codon. To identify cis-acting sequences involved in regulating transcription, HepG2 cells were transfected with vectors containing serial deletions of the promoter-regulatory region of LGP ligated to the cat reporter gene. Two upstream regions were found to enhance transcription. One of these regions contains an alternating purine-pyrimidine sequence. LGP, which lacks a consensus TATA sequence, is like TATA-less and CAAT-less housekeeping genes in that it contains G + C-rich domains upstream from multiple transcription start points. Nuclear proteins from adult rat tissues bound in a tissue-specific fashion to one of these G + C-rich regions.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 203-211 |
| Number of pages | 9 |
| Journal | Gene |
| Volume | 126 |
| Issue number | 2 |
| DOIs | |
| State | Published - Apr 30 1993 |
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Keywords
- alternating purine-pyrimidine
- chloramphenicol acetyltransferase
- Gene expression
- human hepatoma cell line HepG2
- promoter
ASJC Scopus subject areas
- Genetics
Cite this
Characterization of the 5' flanking region of the gene encoding rat liver glycogen phosphorylase. / Herrick, Kevin R.; Gorin, Fredric A; Park, Edwards A.; Tait, Robert C.
In: Gene, Vol. 126, No. 2, 30.04.1993, p. 203-211.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization of the 5' flanking region of the gene encoding rat liver glycogen phosphorylase
AU - Herrick, Kevin R.
AU - Gorin, Fredric A
AU - Park, Edwards A.
AU - Tait, Robert C.
PY - 1993/4/30
Y1 - 1993/4/30
N2 - A genomic region encompassing 800 bp of the promoter-regulatory region and exon 1 of the gene (LGP) encoding rat liver glycogen phosphorylase has been isolated and characterized. Transcripts of the LGP gene initiate predominantly within an 8-bp region 48-bp upstream from the start codon. Additional transcripts were detected that initiate as far as 95 bp upstream from the start codon. To identify cis-acting sequences involved in regulating transcription, HepG2 cells were transfected with vectors containing serial deletions of the promoter-regulatory region of LGP ligated to the cat reporter gene. Two upstream regions were found to enhance transcription. One of these regions contains an alternating purine-pyrimidine sequence. LGP, which lacks a consensus TATA sequence, is like TATA-less and CAAT-less housekeeping genes in that it contains G + C-rich domains upstream from multiple transcription start points. Nuclear proteins from adult rat tissues bound in a tissue-specific fashion to one of these G + C-rich regions.
AB - A genomic region encompassing 800 bp of the promoter-regulatory region and exon 1 of the gene (LGP) encoding rat liver glycogen phosphorylase has been isolated and characterized. Transcripts of the LGP gene initiate predominantly within an 8-bp region 48-bp upstream from the start codon. Additional transcripts were detected that initiate as far as 95 bp upstream from the start codon. To identify cis-acting sequences involved in regulating transcription, HepG2 cells were transfected with vectors containing serial deletions of the promoter-regulatory region of LGP ligated to the cat reporter gene. Two upstream regions were found to enhance transcription. One of these regions contains an alternating purine-pyrimidine sequence. LGP, which lacks a consensus TATA sequence, is like TATA-less and CAAT-less housekeeping genes in that it contains G + C-rich domains upstream from multiple transcription start points. Nuclear proteins from adult rat tissues bound in a tissue-specific fashion to one of these G + C-rich regions.
KW - alternating purine-pyrimidine
KW - chloramphenicol acetyltransferase
KW - Gene expression
KW - human hepatoma cell line HepG2
KW - promoter
UR - http://www.scopus.com/inward/record.url?scp=0027189726&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027189726&partnerID=8YFLogxK
U2 - 10.1016/0378-1119(93)90368-D
DO - 10.1016/0378-1119(93)90368-D
M3 - Article
VL - 126
SP - 203
EP - 211
JO - Gene
JF - Gene
SN - 0378-1119
IS - 2
ER -