Characterization of the 5' flanking region of the gene encoding rat liver glycogen phosphorylase

Kevin R. Herrick, Fredric A Gorin, Edwards A. Park, Robert C. Tait

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A genomic region encompassing 800 bp of the promoter-regulatory region and exon 1 of the gene (LGP) encoding rat liver glycogen phosphorylase has been isolated and characterized. Transcripts of the LGP gene initiate predominantly within an 8-bp region 48-bp upstream from the start codon. Additional transcripts were detected that initiate as far as 95 bp upstream from the start codon. To identify cis-acting sequences involved in regulating transcription, HepG2 cells were transfected with vectors containing serial deletions of the promoter-regulatory region of LGP ligated to the cat reporter gene. Two upstream regions were found to enhance transcription. One of these regions contains an alternating purine-pyrimidine sequence. LGP, which lacks a consensus TATA sequence, is like TATA-less and CAAT-less housekeeping genes in that it contains G + C-rich domains upstream from multiple transcription start points. Nuclear proteins from adult rat tissues bound in a tissue-specific fashion to one of these G + C-rich regions.

Original languageEnglish (US)
Pages (from-to)203-211
Number of pages9
JournalGene
Volume126
Issue number2
DOIs
StatePublished - Apr 30 1993

Fingerprint

Glycogen Phosphorylase
Liver Glycogen
Initiator Codon
5' Flanking Region
Nucleic Acid Regulatory Sequences
Genetic Promoter Regions
Essential Genes
Consensus Sequence
Hep G2 Cells
Nuclear Proteins
Reporter Genes
Genes
Exons
Cats
pyrimidine
purine

Keywords

  • alternating purine-pyrimidine
  • chloramphenicol acetyltransferase
  • Gene expression
  • human hepatoma cell line HepG2
  • promoter

ASJC Scopus subject areas

  • Genetics

Cite this

Characterization of the 5' flanking region of the gene encoding rat liver glycogen phosphorylase. / Herrick, Kevin R.; Gorin, Fredric A; Park, Edwards A.; Tait, Robert C.

In: Gene, Vol. 126, No. 2, 30.04.1993, p. 203-211.

Research output: Contribution to journalArticle

Herrick, Kevin R. ; Gorin, Fredric A ; Park, Edwards A. ; Tait, Robert C. / Characterization of the 5' flanking region of the gene encoding rat liver glycogen phosphorylase. In: Gene. 1993 ; Vol. 126, No. 2. pp. 203-211.
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