Characterization of Th1 activation by Bartonella henselae stimulation in BALB/c mice: Inhibitory activities of interleukin-10 for the production of interferon-γ in spleen cells

Hidenori Kabeya, Akiko Yamasaki, Mikako Ikariya, Rie Negishi, Bruno B Chomel, Soichi Maruyama

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

This study was conducted to analyze cytokine production mechanisms in mice after Bartonella henselae stimulation. BALB/c mice were inoculated intraperitoneally with 3 × 106 colony forming units of B. henselae (Houston-1 strain) twice at 10-day interval. Spleen cells were harvested from the mice and stimulated with the organisms. Following the stimulation, interferon-gamma (IFN-γ) and interleukin-4 (IL-4), IL-10, IL-12 and tumor necrosis factor-alpha (TNF-α) were measured in the culture supernatants of the spleen cells by ELISA. The spleen cells specifically secreted IFN-γ, but not IL-4, indicating that T helper 1 (Th1) cells were activated following B. henselae stimulation. In addition, IL-10 and TNF-α productions were also detected in the culture supernatants of spleen cells. Neutralization of IL-10 in the culture supernatants significantly enhanced the production of IFN-γ from the spleen cells stimulated with B. henselae. These results indicate that B. henselae predominantly stimulated Th1 cells and resulted in secreting IFN-γ, however the production was partially inhibited by IL-10, which was produced simultaneously.

Original languageEnglish (US)
Pages (from-to)290-296
Number of pages7
JournalVeterinary Microbiology
Volume119
Issue number2-4
DOIs
StatePublished - Jan 31 2007

Keywords

  • Bartonella henselae
  • IFN-γ
  • IL-10
  • Mice
  • TNF-α

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Microbiology
  • veterinary(all)

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