Characterization of stress-induced alterations in [3H] Ro5-4864 binding to peripheral benzodiazepine receptors in rat heart and kidney

Robert C. Drugan, Anthony S. Basile, Jacqueline Crawley, Steven M. Paul, Phil Skolnick

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Inescapable tailshock has been shown to elicit a tissue specific decrease in the density of peripheral benzodiazepine receptors (PBR). We have now explored possible mechanisms that may be responsible for this phenomenon. An 80 minute session of inescapable tailshock produced a reduction in the binding of [3H] Ro5-4864 to renal membranes at 0, 1 and 2 hr after stress, with values returning to control (naive) levels within 24 hr. In cardiac membranes, statistically significant reductions were observed only at 2 and 4 hr after stress. The role of the pituitary-adrenal axis and the sympathetic nervous system in this phenomenon was assessed by subjecting adrenalectomized, hypophysectomized, 6-OHDA-treated or control (sham-operated or saline-treated) rats to inescapable shock. Neither adrenalectomy, hypophysectomy, nor 6-OHDA pretreatment altered the stress-induced reduction in renal PBR. However, the stress-induced decrease in renal PBR was blocked by pretreatment with clonazepam (1 mg/kg), a potent anxiolytic with low affinity for PBR.

Original languageEnglish (US)
Pages (from-to)1015-1020
Number of pages6
JournalPharmacology, Biochemistry and Behavior
Volume30
Issue number4
DOIs
StatePublished - 1988
Externally publishedYes

    Fingerprint

Keywords

  • 6-OHDA
  • Adrenalectomy
  • Clonazepam
  • Heart
  • Hypophysectomy
  • Kidney
  • Peripheral benzodiazepine receptors
  • Stress
  • Time course

ASJC Scopus subject areas

  • Biochemistry
  • Behavioral Neuroscience
  • Pharmacology

Cite this