Abstract
Four juvenile rhesus macaques were infected with simian immunodeficiency virus (SIV)(MAC). Freshly isolated peripheral blood mononuclear cells (PBMC) from these SIV(MAC)-infected and from uninfected control macaques were assessed for cytotoxic T-lymphocyte (CTL) activity monthly for 7 consecutive months, beginning 2 months after infection. Target cells consisted of major histocompatibility complex (MHC) haploidentical parental PBMC which were stimulated with mitogen and then pulsed with heat-killed SIV(MAC). CTL activity was demonstrated on all four infected animals. The effector cells are T cells which mediate cytotoxicity against SIV(MAC)-pulsed target cells in an MHC-restricted manner. Furthermore, the cytotoxicity is virus specific and predominantly, if not exclusively, mediated by CD8+ T cells; it is also MHC class-I restricted. Incubation of target cells with leupeptin prior to the cytotoxic assay inhibited target cell generation, suggesting that viral antigens are processed via an endocytic pathway.
Original language | English (US) |
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Pages (from-to) | 785-792 |
Number of pages | 8 |
Journal | AIDS |
Volume | 3 |
Issue number | 12 |
State | Published - 1989 |
Keywords
- AIDS
- Cell-mediated immunity
- cytotoxic T lymphocyte
- simian immunodeficiency virus
ASJC Scopus subject areas
- Immunology
- Immunology and Allergy