Characterization of rice α-amylase isozymes expressed by Saccharomyces cerevisiae

M. Terashima, S. Katoh, B. R. Thomas, R. L. Rodriguez

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Two rice α-amylase isozymes, Amy1A and Amy3D, were produced by secretion from genetically engineered strains of Saccharomyces cerevisiae. They have distinct differences in enzymatic characteristics that can be related to the physiology of the germinating rice seed. The rice isozymes were purified with immunoaffinity chromatography. The pH optima for Amy3D (pH optimum 5.5) and Amy1A (pH optimum 4.2) correlate with the pH of the endosperm tissue at the times in rice seedling development when these isozymes are produced. Amy3D showed 10-14 times higher reactivity to oligosaccharides than Amy1A. Amy1A, on the other hand, showed higher reactivity to soluble starch and starch granules than Amy3D. These results suggest that the isozyme Amy3D, which is expressed at an early stage of germination, produces sugars from soluble starch during the early stage of seed germination and that the isozyme Amy1A works to initiate hydrolysis of the starch granules.

Original languageEnglish (US)
Pages (from-to)1050-1055
Number of pages6
JournalApplied Microbiology and Biotechnology
Volume43
Issue number6
DOIs
StatePublished - 1995

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Amylases
Starch
Yeast
Isoenzymes
Saccharomyces cerevisiae
Seed
Germination
Seeds
Oligosaccharides
Physiology
Chromatography
Endosperm
Sugars
Hydrolysis
Seedlings
Tissue
Oryza

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology
  • Bioengineering

Cite this

Characterization of rice α-amylase isozymes expressed by Saccharomyces cerevisiae. / Terashima, M.; Katoh, S.; Thomas, B. R.; Rodriguez, R. L.

In: Applied Microbiology and Biotechnology, Vol. 43, No. 6, 1995, p. 1050-1055.

Research output: Contribution to journalArticle

Terashima, M. ; Katoh, S. ; Thomas, B. R. ; Rodriguez, R. L. / Characterization of rice α-amylase isozymes expressed by Saccharomyces cerevisiae. In: Applied Microbiology and Biotechnology. 1995 ; Vol. 43, No. 6. pp. 1050-1055.
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