Abstract
Highly enriched rhesus macaque (Macaca mulatta) peripheral blood T-lymphocytes were separated into functional subpopulations by Fc-receptors. The T-lymphocyte population was comprised of both Fc-IgM (Tμ +, 3.4 ± 1.6) and Fc-IgG (Tγ +, 16.2 ± 4.0) bearing cells. T-cells depleted of cells bearing Fc-IgG receptors (Tγ •-) and Tγ + subpopulations were characterized and assessed for functional activity. Tγ + and Tγ •- subpopulations were found to have the following characteristics: 1) Tγ + cells were stimulated by concanavalin-A (Con-A)3, pokeweed mitogen (PWM), and phytohemagglutinin-P (PHA-P), while Tγ •- cells were stimulated by Con-A and PWM, but not PHA-P; 2) Tγ {circled dash} cells were found to mediate PWM induced differentiation of autologous B-cells including EAC+ and EAC- enriched subpopulations, while Tγ + cells did not induce differentiation; 3) Tγ + cells released soluble factors which depressed mitogen stimulation of Tγ {circled dash} cells; and 4) approximately 8-10% of the Tγ + cells phagocytized IgG sensitized bovine red blood cell (BRBC) immune complexes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 517-532 |
| Number of pages | 16 |
| Journal | Veterinary Immunology and Immunopathology |
| Volume | 4 |
| Issue number | 5-6 |
| DOIs | |
| State | Published - Jan 1 1983 |
Fingerprint
ASJC Scopus subject areas
- Immunology
- veterinary(all)
Cite this
Characterization of rhesus macaque peripheral blood T-lymphocyte subpopulations. / Ellingsworth, L. R.; Osburn, Bennie; Hayashi, L. G.; Holmberg, C. A.
In: Veterinary Immunology and Immunopathology, Vol. 4, No. 5-6, 01.01.1983, p. 517-532.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization of rhesus macaque peripheral blood T-lymphocyte subpopulations
AU - Ellingsworth, L. R.
AU - Osburn, Bennie
AU - Hayashi, L. G.
AU - Holmberg, C. A.
PY - 1983/1/1
Y1 - 1983/1/1
N2 - Highly enriched rhesus macaque (Macaca mulatta) peripheral blood T-lymphocytes were separated into functional subpopulations by Fc-receptors. The T-lymphocyte population was comprised of both Fc-IgM (Tμ +, 3.4 ± 1.6) and Fc-IgG (Tγ +, 16.2 ± 4.0) bearing cells. T-cells depleted of cells bearing Fc-IgG receptors (Tγ •-) and Tγ + subpopulations were characterized and assessed for functional activity. Tγ + and Tγ •- subpopulations were found to have the following characteristics: 1) Tγ + cells were stimulated by concanavalin-A (Con-A)3, pokeweed mitogen (PWM), and phytohemagglutinin-P (PHA-P), while Tγ •- cells were stimulated by Con-A and PWM, but not PHA-P; 2) Tγ {circled dash} cells were found to mediate PWM induced differentiation of autologous B-cells including EAC+ and EAC- enriched subpopulations, while Tγ + cells did not induce differentiation; 3) Tγ + cells released soluble factors which depressed mitogen stimulation of Tγ {circled dash} cells; and 4) approximately 8-10% of the Tγ + cells phagocytized IgG sensitized bovine red blood cell (BRBC) immune complexes.
AB - Highly enriched rhesus macaque (Macaca mulatta) peripheral blood T-lymphocytes were separated into functional subpopulations by Fc-receptors. The T-lymphocyte population was comprised of both Fc-IgM (Tμ +, 3.4 ± 1.6) and Fc-IgG (Tγ +, 16.2 ± 4.0) bearing cells. T-cells depleted of cells bearing Fc-IgG receptors (Tγ •-) and Tγ + subpopulations were characterized and assessed for functional activity. Tγ + and Tγ •- subpopulations were found to have the following characteristics: 1) Tγ + cells were stimulated by concanavalin-A (Con-A)3, pokeweed mitogen (PWM), and phytohemagglutinin-P (PHA-P), while Tγ •- cells were stimulated by Con-A and PWM, but not PHA-P; 2) Tγ {circled dash} cells were found to mediate PWM induced differentiation of autologous B-cells including EAC+ and EAC- enriched subpopulations, while Tγ + cells did not induce differentiation; 3) Tγ + cells released soluble factors which depressed mitogen stimulation of Tγ {circled dash} cells; and 4) approximately 8-10% of the Tγ + cells phagocytized IgG sensitized bovine red blood cell (BRBC) immune complexes.
UR - http://www.scopus.com/inward/record.url?scp=0020791740&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0020791740&partnerID=8YFLogxK
U2 - 10.1016/0165-2427(83)90061-2
DO - 10.1016/0165-2427(83)90061-2
M3 - Article
C2 - 6604363
AN - SCOPUS:0020791740
VL - 4
SP - 517
EP - 532
JO - Veterinary Immunology and Immunopathology
JF - Veterinary Immunology and Immunopathology
SN - 0165-2427
IS - 5-6
ER -