Characterization of rhesus macaque peripheral blood T-lymphocyte subpopulations

L. R. Ellingsworth, Bennie Osburn, L. G. Hayashi, C. A. Holmberg

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Highly enriched rhesus macaque (Macaca mulatta) peripheral blood T-lymphocytes were separated into functional subpopulations by Fc-receptors. The T-lymphocyte population was comprised of both Fc-IgM (Tμ +, 3.4 ± 1.6) and Fc-IgG (Tγ +, 16.2 ± 4.0) bearing cells. T-cells depleted of cells bearing Fc-IgG receptors (Tγ •-) and Tγ + subpopulations were characterized and assessed for functional activity. Tγ + and Tγ •- subpopulations were found to have the following characteristics: 1) Tγ + cells were stimulated by concanavalin-A (Con-A)3, pokeweed mitogen (PWM), and phytohemagglutinin-P (PHA-P), while Tγ •- cells were stimulated by Con-A and PWM, but not PHA-P; 2) Tγ {circled dash} cells were found to mediate PWM induced differentiation of autologous B-cells including EAC+ and EAC- enriched subpopulations, while Tγ + cells did not induce differentiation; 3) Tγ + cells released soluble factors which depressed mitogen stimulation of Tγ {circled dash} cells; and 4) approximately 8-10% of the Tγ + cells phagocytized IgG sensitized bovine red blood cell (BRBC) immune complexes.

Original languageEnglish (US)
Pages (from-to)517-532
Number of pages16
JournalVeterinary Immunology and Immunopathology
Volume4
Issue number5-6
DOIs
StatePublished - Jan 1 1983

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Lymphocyte Subsets
Macaca mulatta
T-lymphocytes
T-Lymphocytes
blood
Pokeweed Mitogens
Phytolacca americana
Fc Receptors
Immunoglobulin G
phytohemagglutinin
concanavalin A
Concanavalin A
cells
IgG Receptors
receptors
antigen-antibody complex
Antigen-Antibody Complex
Mitogens
B-lymphocytes
Immunoglobulin M

ASJC Scopus subject areas

  • Immunology
  • veterinary(all)

Cite this

Characterization of rhesus macaque peripheral blood T-lymphocyte subpopulations. / Ellingsworth, L. R.; Osburn, Bennie; Hayashi, L. G.; Holmberg, C. A.

In: Veterinary Immunology and Immunopathology, Vol. 4, No. 5-6, 01.01.1983, p. 517-532.

Research output: Contribution to journalArticle

Ellingsworth, L. R. ; Osburn, Bennie ; Hayashi, L. G. ; Holmberg, C. A. / Characterization of rhesus macaque peripheral blood T-lymphocyte subpopulations. In: Veterinary Immunology and Immunopathology. 1983 ; Vol. 4, No. 5-6. pp. 517-532.
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