Characterization of rhesus macaque B-lymphoblastoid cell lines infected with simian type D retrovirus

R. W. Van Kuyk, R. A. Acevedo, Jose V Torres, N. B. Levy, V. Planelles, R. J. Munn, R. E. Unger, M. B. Gardner, Paul A Luciw

Research output: Contribution to journalArticle

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Abstract

A simian type D retrovirus designated SRV induces a fatal immunosuppressive disease in rhesus macaques. This syndrome shows many clinical similarities to acquired immunodeficiency syndrome (AIDS) in human immunodeficiency virus-infected individuals. To investigate the mechanisms of immune dysfunction in SRV infection, we have focused on the interactions of SRV serotype 1 (SRV-1) with macaque B-lymphoblastoid cell lines (B-LCL). Procedures were optimized for establishing B-LCL by immortalization of macaque B lymphocytes with rhesus Epstein-Barr virus (EBV). These cell lines express B-cell surface markers, secrete immunoglobulins of the IgG or IgM isotypes, and release EBV which transforms monkey B cells. In vitro cultures of B-LCL supported replication of SRV-1. Several B-LCL infected with SRV-1 showed downregulation of major histocompatibility complex (MHC) class II antigen expression whereas levels of MHC class I antigen remained unchanged. Infection of B-LCL with SRV-1 did not alter the level of secreted immunoglobulin. Rhesus EBV was also used to obtain B-LCL from macaques infected with SRV-1; these cell lines were found to release infectious SRV-1. Investigations on the interactions of SRV-1 with B cells will be useful for elucidating mechanisms involved in the immunopathogenesis of primate retroviruses.

Original languageEnglish (US)
Pages (from-to)899-909
Number of pages11
JournalAIDS Research and Human Retroviruses
Volume7
Issue number11
StatePublished - 1991

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Simian Retroviruses
Mason-Pfizer monkey virus
Macaca mulatta
Cell Line
Macaca
B-Lymphocytes
Human Herpesvirus 4
Major Histocompatibility Complex
Immunoglobulins
Histocompatibility Antigens Class I
Histocompatibility Antigens Class II
Retroviridae
Immunosuppressive Agents
Infection
Serogroup
Primates
Haplorhini
Immunoglobulin M
Acquired Immunodeficiency Syndrome
Down-Regulation

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Characterization of rhesus macaque B-lymphoblastoid cell lines infected with simian type D retrovirus. / Van Kuyk, R. W.; Acevedo, R. A.; Torres, Jose V; Levy, N. B.; Planelles, V.; Munn, R. J.; Unger, R. E.; Gardner, M. B.; Luciw, Paul A.

In: AIDS Research and Human Retroviruses, Vol. 7, No. 11, 1991, p. 899-909.

Research output: Contribution to journalArticle

Van Kuyk, RW, Acevedo, RA, Torres, JV, Levy, NB, Planelles, V, Munn, RJ, Unger, RE, Gardner, MB & Luciw, PA 1991, 'Characterization of rhesus macaque B-lymphoblastoid cell lines infected with simian type D retrovirus', AIDS Research and Human Retroviruses, vol. 7, no. 11, pp. 899-909.
Van Kuyk, R. W. ; Acevedo, R. A. ; Torres, Jose V ; Levy, N. B. ; Planelles, V. ; Munn, R. J. ; Unger, R. E. ; Gardner, M. B. ; Luciw, Paul A. / Characterization of rhesus macaque B-lymphoblastoid cell lines infected with simian type D retrovirus. In: AIDS Research and Human Retroviruses. 1991 ; Vol. 7, No. 11. pp. 899-909.
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