Characterization of rat schwannoma-schwann cell hybrids

Barbara Q. Kreider, John Corboy, Sheryl L. Preston, Jeanine M. Auzzmann, Stephanie DeSalvo, Thomas M. Smith, Marge Lieb, Keith Jon Edinburgh, David E Pleasure, F. Arthur McMorris

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Sciatic nerve Schwann cell from strain LEC rats, homozygous for the c form of 6-phosphogluconate dehydrogenase (6-PGD), and RN22 rat Schwannoma cells, a subclone of RN2 deficient in hypoxanthine phosphoribosyltransferase and expressing the s form of 6-PGD, were fused to produce 'RNS' hybrid clones which proloferate rapidly ina medium containing hypoxanthine, aminopterin and thymidine (HAT) and express c, s and c/s heterodimeric forms of 6-PGD. RNS cells, like both parents, maintain a high baseline activity of 2′,3′-cyclic nucleotide 3′-phosphohydrolase and, as in RN22, activity of this enzyme is further inducible by 1 mM N6, O2 -dibutyryl 3′,5′-cyclic AMP. The RNS clones resemble normal Schwann cells in the capacity to bind radioiodinated axolemmal fragments to their plasma membranes.

Original languageEnglish (US)
Pages (from-to)238-244
Number of pages7
JournalBrain Research
Issue number2
StatePublished - Nov 12 1986
Externally publishedYes


  • 2′, 3′ -Cyclic nucleotide 3′ -phosphohydrolase
  • Axolemmal fragment
  • Schwanna cell
  • Schwannoma hybrid

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)


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