Abstract
Sciatic nerve Schwann cell from strain LEC rats, homozygous for the c form of 6-phosphogluconate dehydrogenase (6-PGD), and RN22 rat Schwannoma cells, a subclone of RN2 deficient in hypoxanthine phosphoribosyltransferase and expressing the s form of 6-PGD, were fused to produce 'RNS' hybrid clones which proloferate rapidly ina medium containing hypoxanthine, aminopterin and thymidine (HAT) and express c, s and c/s heterodimeric forms of 6-PGD. RNS cells, like both parents, maintain a high baseline activity of 2′,3′-cyclic nucleotide 3′-phosphohydrolase and, as in RN22, activity of this enzyme is further inducible by 1 mM N6, O2 -dibutyryl 3′,5′-cyclic AMP. The RNS clones resemble normal Schwann cells in the capacity to bind radioiodinated axolemmal fragments to their plasma membranes.
Original language | English (US) |
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Pages (from-to) | 238-244 |
Number of pages | 7 |
Journal | Brain Research |
Volume | 397 |
Issue number | 2 |
DOIs | |
State | Published - Nov 12 1986 |
Externally published | Yes |
Keywords
- 2′, 3′ -Cyclic nucleotide 3′ -phosphohydrolase
- Axolemmal fragment
- Schwanna cell
- Schwannoma hybrid
ASJC Scopus subject areas
- Developmental Biology
- Molecular Biology
- Clinical Neurology
- Neuroscience(all)