Characterization of rat schwannoma-schwann cell hybrids

Barbara Q. Kreider, John Corboy, Sheryl L. Preston, Jeanine M. Auzzmann, Stephanie DeSalvo, Thomas M. Smith, Marge Lieb, Keith Jon Edinburgh, David E Pleasure, F. Arthur McMorris

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Sciatic nerve Schwann cell from strain LEC rats, homozygous for the c form of 6-phosphogluconate dehydrogenase (6-PGD), and RN22 rat Schwannoma cells, a subclone of RN2 deficient in hypoxanthine phosphoribosyltransferase and expressing the s form of 6-PGD, were fused to produce 'RNS' hybrid clones which proloferate rapidly ina medium containing hypoxanthine, aminopterin and thymidine (HAT) and express c, s and c/s heterodimeric forms of 6-PGD. RNS cells, like both parents, maintain a high baseline activity of 2′,3′-cyclic nucleotide 3′-phosphohydrolase and, as in RN22, activity of this enzyme is further inducible by 1 mM N6, O2 -dibutyryl 3′,5′-cyclic AMP. The RNS clones resemble normal Schwann cells in the capacity to bind radioiodinated axolemmal fragments to their plasma membranes.

Original languageEnglish (US)
Pages (from-to)238-244
Number of pages7
JournalBrain Research
Volume397
Issue number2
DOIs
StatePublished - Nov 12 1986
Externally publishedYes

Fingerprint

Phosphogluconate Dehydrogenase
Schwann Cells
Neurilemmoma
nucleotidase
Clone Cells
Aminopterin
Hypoxanthine Phosphoribosyltransferase
Hypoxanthine
Cyclic Nucleotides
Sciatic Nerve
Cyclic AMP
Thymidine
Cell Membrane
Neurons
Enzymes

Keywords

  • 2′, 3′ -Cyclic nucleotide 3′ -phosphohydrolase
  • Axolemmal fragment
  • Schwanna cell
  • Schwannoma hybrid

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Kreider, B. Q., Corboy, J., Preston, S. L., Auzzmann, J. M., DeSalvo, S., Smith, T. M., ... Arthur McMorris, F. (1986). Characterization of rat schwannoma-schwann cell hybrids. Brain Research, 397(2), 238-244. https://doi.org/10.1016/0006-8993(86)90624-4

Characterization of rat schwannoma-schwann cell hybrids. / Kreider, Barbara Q.; Corboy, John; Preston, Sheryl L.; Auzzmann, Jeanine M.; DeSalvo, Stephanie; Smith, Thomas M.; Lieb, Marge; Edinburgh, Keith Jon; Pleasure, David E; Arthur McMorris, F.

In: Brain Research, Vol. 397, No. 2, 12.11.1986, p. 238-244.

Research output: Contribution to journalArticle

Kreider, BQ, Corboy, J, Preston, SL, Auzzmann, JM, DeSalvo, S, Smith, TM, Lieb, M, Edinburgh, KJ, Pleasure, DE & Arthur McMorris, F 1986, 'Characterization of rat schwannoma-schwann cell hybrids', Brain Research, vol. 397, no. 2, pp. 238-244. https://doi.org/10.1016/0006-8993(86)90624-4
Kreider BQ, Corboy J, Preston SL, Auzzmann JM, DeSalvo S, Smith TM et al. Characterization of rat schwannoma-schwann cell hybrids. Brain Research. 1986 Nov 12;397(2):238-244. https://doi.org/10.1016/0006-8993(86)90624-4
Kreider, Barbara Q. ; Corboy, John ; Preston, Sheryl L. ; Auzzmann, Jeanine M. ; DeSalvo, Stephanie ; Smith, Thomas M. ; Lieb, Marge ; Edinburgh, Keith Jon ; Pleasure, David E ; Arthur McMorris, F. / Characterization of rat schwannoma-schwann cell hybrids. In: Brain Research. 1986 ; Vol. 397, No. 2. pp. 238-244.
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