Abstract
Sciatic nerve Schwann cell from strain LEC rats, homozygous for the c form of 6-phosphogluconate dehydrogenase (6-PGD), and RN22 rat Schwannoma cells, a subclone of RN2 deficient in hypoxanthine phosphoribosyltransferase and expressing the s form of 6-PGD, were fused to produce 'RNS' hybrid clones which proloferate rapidly ina medium containing hypoxanthine, aminopterin and thymidine (HAT) and express c, s and c/s heterodimeric forms of 6-PGD. RNS cells, like both parents, maintain a high baseline activity of 2′,3′-cyclic nucleotide 3′-phosphohydrolase and, as in RN22, activity of this enzyme is further inducible by 1 mM N6, O2 -dibutyryl 3′,5′-cyclic AMP. The RNS clones resemble normal Schwann cells in the capacity to bind radioiodinated axolemmal fragments to their plasma membranes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 238-244 |
| Number of pages | 7 |
| Journal | Brain Research |
| Volume | 397 |
| Issue number | 2 |
| DOIs | |
| State | Published - Nov 12 1986 |
| Externally published | Yes |
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Keywords
- 2′, 3′ -Cyclic nucleotide 3′ -phosphohydrolase
- Axolemmal fragment
- Schwanna cell
- Schwannoma hybrid
ASJC Scopus subject areas
- Developmental Biology
- Molecular Biology
- Clinical Neurology
- Neuroscience(all)
Cite this
Characterization of rat schwannoma-schwann cell hybrids. / Kreider, Barbara Q.; Corboy, John; Preston, Sheryl L.; Auzzmann, Jeanine M.; DeSalvo, Stephanie; Smith, Thomas M.; Lieb, Marge; Edinburgh, Keith Jon; Pleasure, David E; Arthur McMorris, F.
In: Brain Research, Vol. 397, No. 2, 12.11.1986, p. 238-244.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization of rat schwannoma-schwann cell hybrids
AU - Kreider, Barbara Q.
AU - Corboy, John
AU - Preston, Sheryl L.
AU - Auzzmann, Jeanine M.
AU - DeSalvo, Stephanie
AU - Smith, Thomas M.
AU - Lieb, Marge
AU - Edinburgh, Keith Jon
AU - Pleasure, David E
AU - Arthur McMorris, F.
PY - 1986/11/12
Y1 - 1986/11/12
N2 - Sciatic nerve Schwann cell from strain LEC rats, homozygous for the c form of 6-phosphogluconate dehydrogenase (6-PGD), and RN22 rat Schwannoma cells, a subclone of RN2 deficient in hypoxanthine phosphoribosyltransferase and expressing the s form of 6-PGD, were fused to produce 'RNS' hybrid clones which proloferate rapidly ina medium containing hypoxanthine, aminopterin and thymidine (HAT) and express c, s and c/s heterodimeric forms of 6-PGD. RNS cells, like both parents, maintain a high baseline activity of 2′,3′-cyclic nucleotide 3′-phosphohydrolase and, as in RN22, activity of this enzyme is further inducible by 1 mM N6, O2 -dibutyryl 3′,5′-cyclic AMP. The RNS clones resemble normal Schwann cells in the capacity to bind radioiodinated axolemmal fragments to their plasma membranes.
AB - Sciatic nerve Schwann cell from strain LEC rats, homozygous for the c form of 6-phosphogluconate dehydrogenase (6-PGD), and RN22 rat Schwannoma cells, a subclone of RN2 deficient in hypoxanthine phosphoribosyltransferase and expressing the s form of 6-PGD, were fused to produce 'RNS' hybrid clones which proloferate rapidly ina medium containing hypoxanthine, aminopterin and thymidine (HAT) and express c, s and c/s heterodimeric forms of 6-PGD. RNS cells, like both parents, maintain a high baseline activity of 2′,3′-cyclic nucleotide 3′-phosphohydrolase and, as in RN22, activity of this enzyme is further inducible by 1 mM N6, O2 -dibutyryl 3′,5′-cyclic AMP. The RNS clones resemble normal Schwann cells in the capacity to bind radioiodinated axolemmal fragments to their plasma membranes.
KW - 2′, 3′ -Cyclic nucleotide 3′ -phosphohydrolase
KW - Axolemmal fragment
KW - Schwanna cell
KW - Schwannoma hybrid
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UR - http://www.scopus.com/inward/citedby.url?scp=0023035703&partnerID=8YFLogxK
U2 - 10.1016/0006-8993(86)90624-4
DO - 10.1016/0006-8993(86)90624-4
M3 - Article
C2 - 3026558
AN - SCOPUS:0023035703
VL - 397
SP - 238
EP - 244
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 2
ER -