Characterization of protein expression levels with label-free detected reverse phase protein arrays

Xuexue Guo, Yihong Deng, Chenggang Zhu, Junlong Cai, Xiangdong Zhu, James P. Landry, Fengyun Zheng, Xunjia Cheng, Yiyan Fei

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

In reverse-phase protein arrays (RPPA), one immobilizes complex samples (e.g., cellular lysate, tissue lysate or serum etc.) on solid supports and performs parallel reactions of antibodies with immobilized protein targets from the complex samples. In this work, we describe a label-free detection of RPPA that enables quantification of RPPA data and thus facilitates comparison of studies performed on different samples and on different solid supports. We applied this detection platform to characterization of phosphoserine aminotransferase (PSAT) expression levels in Acanthamoeba lysates treated with artemether and the results were confirmed by Western blot studies.

Original languageEnglish (US)
Pages (from-to)67-72
Number of pages6
JournalAnalytical Biochemistry
Volume509
DOIs
StatePublished - Sep 15 2016

Keywords

  • Label-free detection
  • Normalization
  • Quantification
  • Reverse phase protein arrays

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Guo, X., Deng, Y., Zhu, C., Cai, J., Zhu, X., Landry, J. P., Zheng, F., Cheng, X., & Fei, Y. (2016). Characterization of protein expression levels with label-free detected reverse phase protein arrays. Analytical Biochemistry, 509, 67-72. https://doi.org/10.1016/j.ab.2016.06.027