Characterization of neutral oligosaccharide-alditols from Xenopus laevis egg jelly coats by matrix-assisted laser desorption Fourier transform mass spectrometry

Ken Tseng, LeAnn L. Lindsay, Sharron Penn, Jerry L. Hedrick, Carlito B Lebrilla

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Neutral oligosaccharides were released by alkaline sodium borohydride reduction of the jelly coating from the South African clawed toad, Xenopus laevis. The oligosaccharides were isolated by HPLC and analyzed by matrix- assisted laser desorption ionization (MALDI)-Fourier transform mass spectrometry (FTMS). The mass spectrometry analysis allowed confirmation of 12 structures first proposed by Strecker et al. using nuclear magnetic resonance. In addition, seven new oligosaccharides with weak abundances were found and characterized by mass spectrometry. A method for discriminating metastable fragments from quasimolecular ions is described. It involves doping the sample with cesium chloride. Cesium-coordinated oligosaccharides do not fragment as readily as those coordinated to sodium. Tandem MS experiments are performed on an unknown oligosaccharide illustrating the potential of MALDI-collision-induced dissociation-FTMS.

Original languageEnglish (US)
Pages (from-to)18-28
Number of pages11
JournalAnalytical Biochemistry
Volume250
Issue number1
DOIs
StatePublished - Jul 15 1997

Fingerprint

Sugar Alcohols
Xenopus laevis
Fourier Analysis
Oligosaccharides
Mass spectrometry
Ovum
Desorption
Mass Spectrometry
Fourier transforms
Lasers
Ionization
Cesium
Anura
Magnetic Resonance Spectroscopy
Sodium
High Pressure Liquid Chromatography
Doping (additives)
Nuclear magnetic resonance
Ions
Coatings

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Characterization of neutral oligosaccharide-alditols from Xenopus laevis egg jelly coats by matrix-assisted laser desorption Fourier transform mass spectrometry. / Tseng, Ken; Lindsay, LeAnn L.; Penn, Sharron; Hedrick, Jerry L.; Lebrilla, Carlito B.

In: Analytical Biochemistry, Vol. 250, No. 1, 15.07.1997, p. 18-28.

Research output: Contribution to journalArticle

Tseng, Ken ; Lindsay, LeAnn L. ; Penn, Sharron ; Hedrick, Jerry L. ; Lebrilla, Carlito B. / Characterization of neutral oligosaccharide-alditols from Xenopus laevis egg jelly coats by matrix-assisted laser desorption Fourier transform mass spectrometry. In: Analytical Biochemistry. 1997 ; Vol. 250, No. 1. pp. 18-28.
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