Characterization of mouse thrombospondin 2 sequence and expression during cell growth and development

Carol D. Laherty, Karen O'Rourke, Frederick W. Wolf, Ronald Katz, Michael F Seldin, Vishva M. Dixit

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98 Scopus citations


Thrombospondin (TSP) is an extracellular matrix glycoprotein whose expression has been associated with a variety of cellular processes including growth and embryogenesis. The recent discovery of the existence of a second mouse TSP gene necessitates careful examination of the discrete biochemical and functional properties associated with each molecule. In this report, the primary structures of human TSP, mouse TSP1 (mTSP1), mouse TSP2 (mTSP2), and chicken TSP are compared; and the expression of mTSP1 and mTSP2 during embryogenesis and growth factor-mediated cell proliferation is examined. The cloning and sequencing of the entire coding regions of mTSP1 and mTSP2 revealed considerable conservation of residues critical for TSP structure and function; these data suggest that TSP2 is capable of trimer formation and many of the same cell-surface and ligand interactions that mediate TSP function. Comparison of the various TSP sequences also allowed the assignment based on sequence homology of previously reported human TSP as TSP1 and chicken TSP as TSP2. mTSP2, like mTSP1, was shown to be a primary response gene when quiescent Swiss 3T3 cells were stimulated with serum, platelet-derived growth factor BB, basic fibroblast growth factor, or interleukin-1β. Interestingly, TSP1 and TSP2 exhibited markedly different tissue-and stage-specific patterns of mRNA expression during mouse embryogenesis, implying that the two TSP molecules possess discrete functional properties important for development. Additionally, the TSP genes (Thbs1 and Thbs2) were mapped to single loci on mouse chromosomes 2 and 17, respectively.

Original languageEnglish (US)
Pages (from-to)3274-3281
Number of pages8
JournalJournal of Biological Chemistry
Issue number5
StatePublished - Feb 15 1992
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry


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