Characterization of mesenchymal stem cells isolated from mouse fetal bone marrow

Xiaoli Wang, Hiroko Hisha, Shigeru Taketani, Yasushi Adachi, Qiang Li, Wenhao Cui, Yunze Cui, Jianfeng Wang, Changye Song, Tomomi Mizokami, Satoshi Okazaki, Qing Li, Tianxue Fan, Hongxue Fan, Zhexiong Lian, M. Eric Gershwin, Susumu Ikehara

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Mesenchymal stem cells (MSCs) are defined as cells that can differentiate into multiple mesenchymal lineage cells. MSCs have some features (surface molecules and cytokine production, etc.) common to so-called traditional bone marrow (BM) stromal cells, which have the capacity to support hemopoiesis. In the present study, we isolated murine MSCs (mMSCs) from the fetal BM using an anti-PA6 monoclonal antibody (mAb) that is specific for bone marrow stromal cells. The mMSCs, called FMS/PA6-P cells, are adherent, fibroblastic, and extensively expanded and have the ability to differentiate not only into osteoblasts and adipocytes but also into vascular endothelial cells. The FMS/PA6-P cells produce a broad spectrum of cytokines and growth factors closely related to hemopoiesis and show good hemopoiesis-supporting capacity both in vivo and in vitro, suggesting that they are a component of the hemopoietic stem cell niche in vivo. Interestingly, although the FMS/PA6-P cells express a high level of the PA6 molecule, which is reactive with anti-PA6 mAb, they gradually lose their ability to express this molecule during the course of differentiation into osteoblasts and adipocytes, indicating that the PA6 molecule might serve as a novel marker of mMSCs.

Original languageEnglish (US)
Pages (from-to)482-493
Number of pages12
JournalStem Cells
Volume24
Issue number3
DOIs
StatePublished - Mar 2006

Fingerprint

Mesenchymal Stromal Cells
Bone Marrow
Osteoblasts
Adipocytes
Monoclonal Antibodies
Cytokines
Stem Cell Niche
Intercellular Signaling Peptides and Proteins
Endothelial Cells

Keywords

  • Bone marrow cells
  • Hematopoietic stem cells
  • Mesenchymal stem cells
  • Mouse
  • PA6

ASJC Scopus subject areas

  • Cell Biology

Cite this

Wang, X., Hisha, H., Taketani, S., Adachi, Y., Li, Q., Cui, W., ... Ikehara, S. (2006). Characterization of mesenchymal stem cells isolated from mouse fetal bone marrow. Stem Cells, 24(3), 482-493. https://doi.org/10.1634/stemcells.2005-0219

Characterization of mesenchymal stem cells isolated from mouse fetal bone marrow. / Wang, Xiaoli; Hisha, Hiroko; Taketani, Shigeru; Adachi, Yasushi; Li, Qiang; Cui, Wenhao; Cui, Yunze; Wang, Jianfeng; Song, Changye; Mizokami, Tomomi; Okazaki, Satoshi; Li, Qing; Fan, Tianxue; Fan, Hongxue; Lian, Zhexiong; Gershwin, M. Eric; Ikehara, Susumu.

In: Stem Cells, Vol. 24, No. 3, 03.2006, p. 482-493.

Research output: Contribution to journalArticle

Wang, X, Hisha, H, Taketani, S, Adachi, Y, Li, Q, Cui, W, Cui, Y, Wang, J, Song, C, Mizokami, T, Okazaki, S, Li, Q, Fan, T, Fan, H, Lian, Z, Gershwin, ME & Ikehara, S 2006, 'Characterization of mesenchymal stem cells isolated from mouse fetal bone marrow', Stem Cells, vol. 24, no. 3, pp. 482-493. https://doi.org/10.1634/stemcells.2005-0219
Wang, Xiaoli ; Hisha, Hiroko ; Taketani, Shigeru ; Adachi, Yasushi ; Li, Qiang ; Cui, Wenhao ; Cui, Yunze ; Wang, Jianfeng ; Song, Changye ; Mizokami, Tomomi ; Okazaki, Satoshi ; Li, Qing ; Fan, Tianxue ; Fan, Hongxue ; Lian, Zhexiong ; Gershwin, M. Eric ; Ikehara, Susumu. / Characterization of mesenchymal stem cells isolated from mouse fetal bone marrow. In: Stem Cells. 2006 ; Vol. 24, No. 3. pp. 482-493.
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