Characterization of horse (Equus caballus) T-cell receptor beta chain genes

Mark D. Schrenzel, Johanna L Watson, David A. Ferrick

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Genes encoding the horse (Equus caballus) T-cell receptor beta chain (TCRB) were cloned and characterized. Of 33 cDNA clones isolated from the mesenteric lymph node, 30 had functionally rearranged gene segments, and three contained germline sequences. Sixteen unique variable segments (TCRBV), 14 joining genes (TCRBJ), and two constant region genes (TCRBC) were identified. Horse TCRBV were grouped into nine families based on similarity to human sequences. TCRBV2 and TCRBV12 were the most commonly represented horse families. Analysis of predicted protein structure revealed the presence of conserved regions similar to those seen in TCRB of other species. A decanucleotide promoter sequence homologous to those found in humans and mice was located in the 5′ untranslated region of one horse gene. Germline sequences included the 5′ region of the TCRBD2 gene with flanking heptamer/nonamer recombination signals and portions of the TCRBJ-C2 intron. Southern blot hybridizations demonstrated restriction fragment length polymorphisms at the TCRBC locus among different horse breeds.

Original languageEnglish (US)
Pages (from-to)135-144
Number of pages10
JournalImmunogenetics
Volume40
Issue number2
DOIs
StatePublished - Jun 1994

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T-Cell Receptor beta Genes
Horses
Genes
Antigen Receptors, T-Cell, alpha-beta
5' Untranslated Regions
Sequence Homology
Southern Blotting
Restriction Fragment Length Polymorphisms
Introns
Genetic Recombination
Complementary DNA
Clone Cells
Lymph Nodes

ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this

Characterization of horse (Equus caballus) T-cell receptor beta chain genes. / Schrenzel, Mark D.; Watson, Johanna L; Ferrick, David A.

In: Immunogenetics, Vol. 40, No. 2, 06.1994, p. 135-144.

Research output: Contribution to journalArticle

Schrenzel, Mark D. ; Watson, Johanna L ; Ferrick, David A. / Characterization of horse (Equus caballus) T-cell receptor beta chain genes. In: Immunogenetics. 1994 ; Vol. 40, No. 2. pp. 135-144.
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