Characterization of HIV-1-specific cytotoxic T lymphocytes expressing the mucosal lymphocyte integrin CD103 in rectal and duodenal lymphoid tissue of HIV-1-infected subjects

Barbara Shacklett, Thomas J. Beadle, Paulo A. Pacheco, James H. Grendell, Patrick A J Haslett, Abigail S. King, Graham S. Ogg, Paul M. Basuk, Douglas F. Nixon

Research output: Contribution to journalArticle

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Abstract

Acute HIV-1 infection depletes CD4+ T cells in gut-associated lymphoid tissue (GALT). The failure of containment of local viral replication, and consequent CD4+ T cell depletion, might be due to delayed mobilization of effector CD8+ T cells or absence of functioning HIV-1-specific CD8+ T cell effectors within GALT. No studies have addressed human intestinal HIV-1- specific CD8+ T cell functions. We sought to determine whether functional HIV-1-specific CTL were present in GALT and whether the repertoire differed from HIV-1-specific CTL isolated from peripheral blood mononuclear cells. From three HIV-1-infected subjects, we isolated HIV-1-specific CD8+ T cells expressing the mucosal lymphocyte integrin CD103 from GALT. These antigen- specific effector cells could be expanded in vitro and lysed target cells in an MHC class I-restricted manner. HIV-1-specific CTL could be isolated from both duodenal and rectal GALT sites, indicating that CD8+ effectors were widespread through GALT tissue. The breadth and antigenic specificities of GALT CTL appeared to differ from those in peripheral blood in some cases. In summary, we found HIV-1-specific CD8+ effector T cells in GALT, despite HIV- 1-induced CD4+ T cell lymphopenia. This suggests that HIV-1-specific CTL in gut tissue can be maintained with limited CD4+ T cell help. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)317-327
Number of pages11
JournalVirology
Volume270
Issue number2
DOIs
StatePublished - May 10 2000
Externally publishedYes

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Cytotoxic T-Lymphocytes
Lymphoid Tissue
Integrins
HIV-1
Lymphocytes
T-Lymphocytes
Lymphopenia
HIV Infections
Epitopes
Blood Cells
Antigens

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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Characterization of HIV-1-specific cytotoxic T lymphocytes expressing the mucosal lymphocyte integrin CD103 in rectal and duodenal lymphoid tissue of HIV-1-infected subjects. / Shacklett, Barbara; Beadle, Thomas J.; Pacheco, Paulo A.; Grendell, James H.; Haslett, Patrick A J; King, Abigail S.; Ogg, Graham S.; Basuk, Paul M.; Nixon, Douglas F.

In: Virology, Vol. 270, No. 2, 10.05.2000, p. 317-327.

Research output: Contribution to journalArticle

Shacklett, Barbara ; Beadle, Thomas J. ; Pacheco, Paulo A. ; Grendell, James H. ; Haslett, Patrick A J ; King, Abigail S. ; Ogg, Graham S. ; Basuk, Paul M. ; Nixon, Douglas F. / Characterization of HIV-1-specific cytotoxic T lymphocytes expressing the mucosal lymphocyte integrin CD103 in rectal and duodenal lymphoid tissue of HIV-1-infected subjects. In: Virology. 2000 ; Vol. 270, No. 2. pp. 317-327.
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