Characterization of francisella species isolated from the cooling water of an air conditioning system

Quan Gu, Xunde Li, Pinghua Qu, Shuiping Hou, Juntao Li, Edward R Atwill, Shouyi Chen

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Strains of Francisella spp. were isolated from cooling water from an air conditioning system in Guangzhou, China. These strains are Gram negative, coccobacilli, non-motile, oxidase negative, catalase negative, esterase and lipid esterase positive. In addition, these bacteria grow on cysteine-supplemented media at 20 °C to 40 °C with an optimal growth temperature of 30 °C. Analysis of 16S rRNA gene sequences revealed that these strains belong to the genus Francisella. Biochemical tests and phylogenetic and BLAST analyses of 16S rRNA, rpoB and sdhA genes indicated that one strain was very similar to Francisellaphilomiragia and that the other strains were identical or highly similar to the Francisella guangzhouensis sp. nov. strain 08HL01032 we previously described. Biochemical and molecular characteristics of these strains demonstrated that multiple Francisella species exist in air conditioning systems.

Original languageEnglish (US)
Pages (from-to)921-927
Number of pages7
JournalBrazilian Journal of Microbiology
Volume46
Issue number3
DOIs
StatePublished - Jul 1 2015

    Fingerprint

Keywords

  • Air conditioning
  • Francisella
  • Water

ASJC Scopus subject areas

  • Microbiology

Cite this