Characterization of four polymorphic genes controlling red leaf colour in lettuce that have undergone disruptive selection since domestication

Wenqing Su, Rong Tao, Wenye Liu, Changchun Yu, Zhen Yue, Shuping He, Dean Lavelle, Weiyi Zhang, Lei Zhang, Guanghui An, Yu Zhang, Qun Hu, Robert M. Larkin, Richard W Michelmore, Hanhui Kuang, Jiongjiong Chen

Research output: Contribution to journalArticle

Abstract

Anthocyanins protect plants from biotic and abiotic stressors and provide great health benefits to consumers. In this study, we cloned four genes (Red Lettuce Leaves 1 to 4: RLL1 to RLL4) that contribute to colour variations in lettuce. The RLL1 gene encodes a bHLH transcription factor, and a 5-bp deletion in some cultivars abolishes its function to activate the anthocyanin biosynthesis pathway. The RLL2 gene encodes an R2R3-MYB transcription factor, which was derived from a duplication followed by mutations in its promoter region. The RLL3 gene encodes an R2-MYB transcription factor, which down-regulates anthocyanin biosynthesis through competing with RLL2 for interaction with RLL1; a mis-sense mutation compromises the capacity of RLL3 to bind RLL1. The RLL4 gene encodes a WD-40 transcription factor, homologous to the RUP genes suppressing the UV-B signal transduction pathway in Arabidopsis; a mis-sense mutation in rll4 attenuates its suppressing function, leading to a high concentration of anthocyanins. Sequence analysis of the RLL1-RLL4 genes from wild and cultivated lettuce showed that their function-changing mutations occurred after domestication. The mutations in rll1 disrupt anthocyanin biosynthesis, while the mutations in RLL2, rll3 and rll4 activate anthocyanin biosynthesis, showing disruptive selection for leaf colour during domestication of lettuce. The characterization of multiple polymorphic genes in this study provides the necessary molecular resources for the rational breeding of lettuce cultivars with distinct levels of red pigments and green cultivars with high levels of health-promoting flavonoids.

Original languageEnglish (US)
JournalPlant Biotechnology Journal
DOIs
StateAccepted/In press - Jan 1 2019

Fingerprint

Lettuce
domestication
lettuce
Anthocyanins
Color
anthocyanins
color
Mutation
Genes
leaves
genes
transcription factors
biosynthesis
mutation
missense mutation
Transcription Factors
cultivars
Basic Helix-Loop-Helix Transcription Factors
leaf lettuce
health promotion

Keywords

  • anthocyanin
  • bulked segregant analysis
  • disruptive selection
  • leaf colour
  • lettuce
  • QTL-seq

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science

Cite this

Characterization of four polymorphic genes controlling red leaf colour in lettuce that have undergone disruptive selection since domestication. / Su, Wenqing; Tao, Rong; Liu, Wenye; Yu, Changchun; Yue, Zhen; He, Shuping; Lavelle, Dean; Zhang, Weiyi; Zhang, Lei; An, Guanghui; Zhang, Yu; Hu, Qun; Larkin, Robert M.; Michelmore, Richard W; Kuang, Hanhui; Chen, Jiongjiong.

In: Plant Biotechnology Journal, 01.01.2019.

Research output: Contribution to journalArticle

Su, W, Tao, R, Liu, W, Yu, C, Yue, Z, He, S, Lavelle, D, Zhang, W, Zhang, L, An, G, Zhang, Y, Hu, Q, Larkin, RM, Michelmore, RW, Kuang, H & Chen, J 2019, 'Characterization of four polymorphic genes controlling red leaf colour in lettuce that have undergone disruptive selection since domestication', Plant Biotechnology Journal. https://doi.org/10.1111/pbi.13213
Su, Wenqing ; Tao, Rong ; Liu, Wenye ; Yu, Changchun ; Yue, Zhen ; He, Shuping ; Lavelle, Dean ; Zhang, Weiyi ; Zhang, Lei ; An, Guanghui ; Zhang, Yu ; Hu, Qun ; Larkin, Robert M. ; Michelmore, Richard W ; Kuang, Hanhui ; Chen, Jiongjiong. / Characterization of four polymorphic genes controlling red leaf colour in lettuce that have undergone disruptive selection since domestication. In: Plant Biotechnology Journal. 2019.
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AU - Yu, Changchun

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AU - He, Shuping

AU - Lavelle, Dean

AU - Zhang, Weiyi

AU - Zhang, Lei

AU - An, Guanghui

AU - Zhang, Yu

AU - Hu, Qun

AU - Larkin, Robert M.

AU - Michelmore, Richard W

AU - Kuang, Hanhui

AU - Chen, Jiongjiong

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