Characterization of feline CD56 molecule expressed on insect cells by the baculovirus expression system

Yorihiro Nishimura, Takayuki Miyazawa, Yasuhiro Ikeda, Yoshihiro Izumiya, Kazuya Nakamura, Jin Shun Cai, Eiji Sato, Mariko Kohmoto, Takeshi Mikami, Eiji Takahashi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Recently we cloned 140 kDa form of feline CD56 cDNA. In this study, we expressed the feline CD56 molecule by the baculovirus expression system. We found that the molecule was expressed on the cell surface when examined by the indirect immunofluorescence assay using an anti-human CD56 monoclonal antibody. Immunoblotting analysis revealed that the molecular weight of the major expressed product was 140 kDa. Interestingly we found that the insect cells expressing the feline CD56 molecule aggregated, indicating that the expressed molecule mediates homophilic adhesion.

Original languageEnglish (US)
Pages (from-to)701-703
Number of pages3
JournalJournal of Veterinary Medical Science
Volume61
Issue number6
StatePublished - Jun 1999
Externally publishedYes

Fingerprint

Baculoviridae
Felidae
Insects
cats
insects
cells
Indirect Fluorescent Antibody Technique
immunoblotting
Immunoblotting
fluorescent antibody technique
adhesion
monoclonal antibodies
Complementary DNA
Molecular Weight
Monoclonal Antibodies
molecular weight
assays
Feline
Baculovirus

Keywords

  • CD56
  • Expression
  • Feline

ASJC Scopus subject areas

  • veterinary(all)
  • Marketing

Cite this

Nishimura, Y., Miyazawa, T., Ikeda, Y., Izumiya, Y., Nakamura, K., Cai, J. S., ... Takahashi, E. (1999). Characterization of feline CD56 molecule expressed on insect cells by the baculovirus expression system. Journal of Veterinary Medical Science, 61(6), 701-703.

Characterization of feline CD56 molecule expressed on insect cells by the baculovirus expression system. / Nishimura, Yorihiro; Miyazawa, Takayuki; Ikeda, Yasuhiro; Izumiya, Yoshihiro; Nakamura, Kazuya; Cai, Jin Shun; Sato, Eiji; Kohmoto, Mariko; Mikami, Takeshi; Takahashi, Eiji.

In: Journal of Veterinary Medical Science, Vol. 61, No. 6, 06.1999, p. 701-703.

Research output: Contribution to journalArticle

Nishimura, Y, Miyazawa, T, Ikeda, Y, Izumiya, Y, Nakamura, K, Cai, JS, Sato, E, Kohmoto, M, Mikami, T & Takahashi, E 1999, 'Characterization of feline CD56 molecule expressed on insect cells by the baculovirus expression system', Journal of Veterinary Medical Science, vol. 61, no. 6, pp. 701-703.
Nishimura, Yorihiro ; Miyazawa, Takayuki ; Ikeda, Yasuhiro ; Izumiya, Yoshihiro ; Nakamura, Kazuya ; Cai, Jin Shun ; Sato, Eiji ; Kohmoto, Mariko ; Mikami, Takeshi ; Takahashi, Eiji. / Characterization of feline CD56 molecule expressed on insect cells by the baculovirus expression system. In: Journal of Veterinary Medical Science. 1999 ; Vol. 61, No. 6. pp. 701-703.
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