Abstract
The specificity of F21.A, a monoclonal antibody raised against bottlenose dolphin leucocytes, was characterized in killer whale on the basis of immunoprecipitation of a protein of 94kDa, as well as flow cytometric analysis. While minimally expressed on resting cells, F21.A labeled a homologue to β-2 integrin in 89-97% of PMA-activated neutrophils, 53-66% of activated monocytes, and activated B cells but not T cells. Activation of neutrophils reached its maximum 10min after PMA stimulation. F21.A did not label intracellular stores as did both cross-reacting anti-canine CD11b and CD18, suggesting that an activation-induced conformational change would expose a neoepitope recognized by F21.A. F21.A labeling was largely inhibited by pre-incubation with plasma, suggesting a binding site closely related to that for fibrinogen. In vitro phagocytosis and respiratory burst were almost fully inhibited upon pre-incubation with F21.A, demonstrating its functional importance. This antibody is foreseen as a possible valuable diagnostic and research tool in cetacean immunology.
Original language | English (US) |
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Pages (from-to) | 195-206 |
Number of pages | 12 |
Journal | Veterinary Immunology and Immunopathology |
Volume | 97 |
Issue number | 3-4 |
DOIs | |
State | Published - Feb 2004 |
Keywords
- β-2 integrin
- CD11
- CD18
- Cetaceans
- Immunology
- Phenotyping
ASJC Scopus subject areas
- Animal Science and Zoology
- Immunology
- veterinary(all)