Characterization of F21.A, a monoclonal antibody that recognize a leucocyte surface antigen for killer whale homologue to β-2 integrin

S. De Guise, K. Erickson, Myra Blanchard, L. DiMolfetto, H. D. Lepper, Jeffrey L Stott, D. A. Ferrick

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

The specificity of F21.A, a monoclonal antibody raised against bottlenose dolphin leucocytes, was characterized in killer whale on the basis of immunoprecipitation of a protein of 94kDa, as well as flow cytometric analysis. While minimally expressed on resting cells, F21.A labeled a homologue to β-2 integrin in 89-97% of PMA-activated neutrophils, 53-66% of activated monocytes, and activated B cells but not T cells. Activation of neutrophils reached its maximum 10min after PMA stimulation. F21.A did not label intracellular stores as did both cross-reacting anti-canine CD11b and CD18, suggesting that an activation-induced conformational change would expose a neoepitope recognized by F21.A. F21.A labeling was largely inhibited by pre-incubation with plasma, suggesting a binding site closely related to that for fibrinogen. In vitro phagocytosis and respiratory burst were almost fully inhibited upon pre-incubation with F21.A, demonstrating its functional importance. This antibody is foreseen as a possible valuable diagnostic and research tool in cetacean immunology.

Original languageEnglish (US)
Pages (from-to)195-206
Number of pages12
JournalVeterinary Immunology and Immunopathology
Volume97
Issue number3-4
DOIs
StatePublished - Feb 2004

Keywords

  • β-2 integrin
  • CD11
  • CD18
  • Cetaceans
  • Immunology
  • Phenotyping

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Immunology
  • veterinary(all)

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