Characterization of equine intestinal fatty acid binding protein and its use in managing horses with colic

Jorge Nieto, Brian M. Aldridge, Pablo M. Beldomenico, Monica R Aleman, Jack R. Snyder

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Objective - To determine the nucleotide sequence of the equine intestinal fatty acid binding protein (I-FABP) gene, its expression in various regions of the gastrointestinal tract, and the use of measuring I-FABP in horses with colic. Animals - 86 horses with colic. Procedure - The mRNA sequence for the I-FABP gene was obtained by use of a rapid amplification of complementary DNA ends technique. Comparative I-FABP gene expression was quantitated by use of a real-time reverse transcription-polymerase chain reaction assay. Amounts of I-FABP in abdominal fluid and plasma were measured by use of an ELISA kit. Association between I-FABP concentrations and clinical variables was performed by nonparametric analysis, and associations of these variables with intestinal ischemia were determined by the Spearman correlation test. Results - The nucleotide sequence had 87% identity with human I-FABP. The I-FABP gene was highly expressed in the small intestinal mucosa but had low expression in the colon. High concentrations of I-FABP in abdominal fluid correlated with an increase in protein concentrations in peritoneal fluid and non-survival, whereas plasma I-FABP concentrations correlated with the necessity for abdominal surgery. Clinical variables associated with intestinal ischemia included the color and protein content of abdominal fluid and serum creatine kinase activity. Conclusions and Clinical Relevance - Determination of I-FABP concentrations in abdominal fluid and plasma may be useful for predicting survival and the need for abdominal surgical intervention in horses with colic. Furthermore, serum creatine kinase activity and color and protein concentrations of abdominal fluid may be useful in the diagnosis of intestinal ischemia.

Original languageEnglish (US)
Pages (from-to)223-232
Number of pages10
JournalAmerican Journal of Veterinary Research
Volume66
Issue number2
DOIs
StatePublished - Feb 2005

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fatty acid-binding proteins
Fatty Acid-Binding Proteins
Colic
colic
Horses
horses
ischemia
Ischemia
creatine kinase
Creatine Kinase
Color
Gene Expression
nucleotide sequences
gene expression
complementary DNA
Proteins
color
Ascitic Fluid
Intestinal Mucosa
intestinal mucosa

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Characterization of equine intestinal fatty acid binding protein and its use in managing horses with colic. / Nieto, Jorge; Aldridge, Brian M.; Beldomenico, Pablo M.; Aleman, Monica R; Snyder, Jack R.

In: American Journal of Veterinary Research, Vol. 66, No. 2, 02.2005, p. 223-232.

Research output: Contribution to journalArticle

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