Characterization of equine E-selectin

Jodi F. Hedges, Christopher D. Demaula, Brian D. Moore, Bridget E. Mclaughlin, Scott I. Simon, Nigel J Maclachlan

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Expression of E-selectin on activated endothelium is a critical initial step that leads to extravasation of leucocytes during inflammation, yet E-selectin is largely uncharacterized in several animal species including the horse. We have sequenced and compared E-selectin genes derived from activated cultures of purified equine (horse), cervid (black-tailed deer) and ovine (sheep) pulmonary artery endothelial cells (ECs). Phylogenetic and amino acid sequence comparisons indicate that bovine, cervid and ovine E-selectin are similar, whereas human and equine E-selectin are more closely related to each other than to the ruminant molecules. Human E- and P-selectin-specific monoclonal antibodies that also recognize equine E-selectin were identified and used to characterize its expression. Expression of E-selectin was more readily induced by lipopolysaccharide treatment in equine ECs than in human ECs and supported adhesion and activation of neutrophils, consistent with the extreme sensitivity of horses to endotoxaemia and septic shock.

Original languageEnglish (US)
Pages (from-to)498-504
Number of pages7
JournalImmunology
Volume103
Issue number4
DOIs
StatePublished - 2001

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E-Selectin
Horses
Sheep
Endothelial Cells
Neutrophil Activation
Endotoxemia
Deer
Ruminants
Septic Shock
Cell Adhesion
Pulmonary Artery
Endothelium
Lipopolysaccharides
Amino Acid Sequence
Leukocytes
Monoclonal Antibodies
Inflammation
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

Hedges, J. F., Demaula, C. D., Moore, B. D., Mclaughlin, B. E., Simon, S. I., & Maclachlan, N. J. (2001). Characterization of equine E-selectin. Immunology, 103(4), 498-504. https://doi.org/10.1046/j.1365-2567.2001.01262.x

Characterization of equine E-selectin. / Hedges, Jodi F.; Demaula, Christopher D.; Moore, Brian D.; Mclaughlin, Bridget E.; Simon, Scott I.; Maclachlan, Nigel J.

In: Immunology, Vol. 103, No. 4, 2001, p. 498-504.

Research output: Contribution to journalArticle

Hedges, JF, Demaula, CD, Moore, BD, Mclaughlin, BE, Simon, SI & Maclachlan, NJ 2001, 'Characterization of equine E-selectin', Immunology, vol. 103, no. 4, pp. 498-504. https://doi.org/10.1046/j.1365-2567.2001.01262.x
Hedges JF, Demaula CD, Moore BD, Mclaughlin BE, Simon SI, Maclachlan NJ. Characterization of equine E-selectin. Immunology. 2001;103(4):498-504. https://doi.org/10.1046/j.1365-2567.2001.01262.x
Hedges, Jodi F. ; Demaula, Christopher D. ; Moore, Brian D. ; Mclaughlin, Bridget E. ; Simon, Scott I. ; Maclachlan, Nigel J. / Characterization of equine E-selectin. In: Immunology. 2001 ; Vol. 103, No. 4. pp. 498-504.
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