Abstract
Expression of E-selectin on activated endothelium is a critical initial step that leads to extravasation of leucocytes during inflammation, yet E-selectin is largely uncharacterized in several animal species including the horse. We have sequenced and compared E-selectin genes derived from activated cultures of purified equine (horse), cervid (black-tailed deer) and ovine (sheep) pulmonary artery endothelial cells (ECs). Phylogenetic and amino acid sequence comparisons indicate that bovine, cervid and ovine E-selectin are similar, whereas human and equine E-selectin are more closely related to each other than to the ruminant molecules. Human E- and P-selectin-specific monoclonal antibodies that also recognize equine E-selectin were identified and used to characterize its expression. Expression of E-selectin was more readily induced by lipopolysaccharide treatment in equine ECs than in human ECs and supported adhesion and activation of neutrophils, consistent with the extreme sensitivity of horses to endotoxaemia and septic shock.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 498-504 |
| Number of pages | 7 |
| Journal | Immunology |
| Volume | 103 |
| Issue number | 4 |
| DOIs | |
| State | Published - 2001 |
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ASJC Scopus subject areas
- Immunology
Cite this
Characterization of equine E-selectin. / Hedges, Jodi F.; Demaula, Christopher D.; Moore, Brian D.; Mclaughlin, Bridget E.; Simon, Scott I.; Maclachlan, Nigel J.
In: Immunology, Vol. 103, No. 4, 2001, p. 498-504.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization of equine E-selectin
AU - Hedges, Jodi F.
AU - Demaula, Christopher D.
AU - Moore, Brian D.
AU - Mclaughlin, Bridget E.
AU - Simon, Scott I.
AU - Maclachlan, Nigel J
PY - 2001
Y1 - 2001
N2 - Expression of E-selectin on activated endothelium is a critical initial step that leads to extravasation of leucocytes during inflammation, yet E-selectin is largely uncharacterized in several animal species including the horse. We have sequenced and compared E-selectin genes derived from activated cultures of purified equine (horse), cervid (black-tailed deer) and ovine (sheep) pulmonary artery endothelial cells (ECs). Phylogenetic and amino acid sequence comparisons indicate that bovine, cervid and ovine E-selectin are similar, whereas human and equine E-selectin are more closely related to each other than to the ruminant molecules. Human E- and P-selectin-specific monoclonal antibodies that also recognize equine E-selectin were identified and used to characterize its expression. Expression of E-selectin was more readily induced by lipopolysaccharide treatment in equine ECs than in human ECs and supported adhesion and activation of neutrophils, consistent with the extreme sensitivity of horses to endotoxaemia and septic shock.
AB - Expression of E-selectin on activated endothelium is a critical initial step that leads to extravasation of leucocytes during inflammation, yet E-selectin is largely uncharacterized in several animal species including the horse. We have sequenced and compared E-selectin genes derived from activated cultures of purified equine (horse), cervid (black-tailed deer) and ovine (sheep) pulmonary artery endothelial cells (ECs). Phylogenetic and amino acid sequence comparisons indicate that bovine, cervid and ovine E-selectin are similar, whereas human and equine E-selectin are more closely related to each other than to the ruminant molecules. Human E- and P-selectin-specific monoclonal antibodies that also recognize equine E-selectin were identified and used to characterize its expression. Expression of E-selectin was more readily induced by lipopolysaccharide treatment in equine ECs than in human ECs and supported adhesion and activation of neutrophils, consistent with the extreme sensitivity of horses to endotoxaemia and septic shock.
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UR - http://www.scopus.com/inward/citedby.url?scp=0034885116&partnerID=8YFLogxK
U2 - 10.1046/j.1365-2567.2001.01262.x
DO - 10.1046/j.1365-2567.2001.01262.x
M3 - Article
C2 - 11529941
AN - SCOPUS:0034885116
VL - 103
SP - 498
EP - 504
JO - Immunology
JF - Immunology
SN - 0019-2805
IS - 4
ER -