Characterization of equine adipose tissue-derived stromal cells

Adipogenic and osteogenic capacity and comparison with bone marrow-derived mesenchymal stromal cells

Martin A. Vidal, Gail E. Kilroy, Mandi J. Lopez, Jill R. Johnson, Rustin M. Moore, Jeffrey M. Gimble

Research output: Contribution to journalArticle

167 Citations (Scopus)

Abstract

To characterize equine adipose tissue-derived stromal cell (ASC) frequency and growth characteristics and assess of their adipogenic and osteogenic differentiation potential. In vitro experimental study. Horses (n=5; aged, 9 months to 5 years). Cell doubling characteristics of ASCs harvested from supragluteal subcutaneous adipose tissue were evaluated over 10 passages. Primary, second (P2), and fourth (P4) passage ASCs were induced under appropriate conditions to undergo adipogenesis and osteogenesis. Limit dilution assays were performed on each passage to determine the frequency of colony-forming units with a fibroblastic (CFU-F) phenotype and the frequency of ASC differentiation into the adipocyte (CFU-Ad) and osteoblast (CFU-Ob) phenotype. ASC isolates exhibited an average cell-doubling time of 2.1±0.9 days during the first 10 cell doublings. Approximately 1 in 2.3±0.4 of the total stromal vascular fraction nucleated cells were ASCs, based on the CFU-F assays, and 1 in 3.6±1.3 expressed alkaline phosphatase, an osteogenic marker. Primary ASCs differentiated in response to adipogenic (1 in 4.9±5.4, CFU-Ad) and osteogenic (1 in <2.44, CFU-Ob) inductive conditions and maintained their differentiation potential during subsequent passages (P2 and P4). The frequency, in vitro growth rate, and adipogenic and osteogenic differentiation potential of equine ASCs show some differences to those documented for ASCs in other mammalian species. Adipose tissue is a potential source of adult stem cells for tissue engineering applications in equine veterinary medicine.

Original languageEnglish (US)
Pages (from-to)613-622
Number of pages10
JournalVeterinary Surgery
Volume36
Issue number7
DOIs
StatePublished - Oct 2007
Externally publishedYes

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stromal cells
Stromal Cells
Mesenchymal Stromal Cells
bone marrow
adipose tissue
Horses
Adipose Tissue
Bone Marrow
horses
osteoblasts
Osteoblasts
Stem Cells
cell engineering
cells
Cell Engineering
Phenotype
phenotype
tissue engineering
Adipogenesis
Adult Stem Cells

ASJC Scopus subject areas

  • veterinary(all)

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Characterization of equine adipose tissue-derived stromal cells : Adipogenic and osteogenic capacity and comparison with bone marrow-derived mesenchymal stromal cells. / Vidal, Martin A.; Kilroy, Gail E.; Lopez, Mandi J.; Johnson, Jill R.; Moore, Rustin M.; Gimble, Jeffrey M.

In: Veterinary Surgery, Vol. 36, No. 7, 10.2007, p. 613-622.

Research output: Contribution to journalArticle

Vidal, Martin A. ; Kilroy, Gail E. ; Lopez, Mandi J. ; Johnson, Jill R. ; Moore, Rustin M. ; Gimble, Jeffrey M. / Characterization of equine adipose tissue-derived stromal cells : Adipogenic and osteogenic capacity and comparison with bone marrow-derived mesenchymal stromal cells. In: Veterinary Surgery. 2007 ; Vol. 36, No. 7. pp. 613-622.
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