Characterization of densin-180, a new brain-specific synaptic protein of the O-sialoglycoprotein family

Michelle L Apperson, Il Soo Moon, Mary B. Kennedy

Research output: Contribution to journalArticle

129 Citations (Scopus)

Abstract

We purified an abundant protein of apparent molecular mass 180 kDa from the postsynaptic density fraction of rat forebrain and obtained amino acid sequences of three tryptic peptides generated from the protein. The sequences were used to design a strategy for cloning the cDNA encoding the protein by polymerase chain reaction. The open reading frame of the cDNA encodes a novel protein of predicted molecular mass 167 kDa. We have named the protein densin-180. Antibodies raised against the predicted amino and carboxyl sequences of densin-180 recognize a 180 kDa band on immunoblots that is enriched in the postsynaptic density fraction. Immunocytochemical localization of densin-180 in dissociated hippocampal neuronal cultures shows that the protein is highly concentrated at synapses along dendrites. The message encoding densin-180 is brain specific and is more abundant in forebrain than in cerebellum. The sequence of densin-180 contains 17 leucine- rich repeats, a sialomucin domain, an apparent transmembrane domain, and a PDZ domain. This arrangement of domains is similar to that of several adhesion molecules, in particular GPIbα, which mediates binding of platelets to von Willebrand factor. We propose that densin-180 participates in specific adhesion between presynaptic and postsynaptic membranes at glutamatergic synapses.

Original languageEnglish (US)
Pages (from-to)6839-6852
Number of pages14
JournalJournal of Neuroscience
Volume16
Issue number21
StatePublished - Nov 1 1996
Externally publishedYes

Fingerprint

Sialoglycoproteins
Brain
Post-Synaptic Density
Proteins
Prosencephalon
Synapses
Complementary DNA
Sialomucins
PDZ Domains
von Willebrand Factor
Dendrites
Leucine
Cerebellum
Open Reading Frames
Organism Cloning
Amino Acid Sequence
Blood Platelets
Polymerase Chain Reaction
Peptides
Membranes

Keywords

  • adhesion molecule
  • microsequencing
  • polymerase chain reaction
  • postsynaptic density
  • synapse development
  • synaptic cleft

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Characterization of densin-180, a new brain-specific synaptic protein of the O-sialoglycoprotein family. / Apperson, Michelle L; Moon, Il Soo; Kennedy, Mary B.

In: Journal of Neuroscience, Vol. 16, No. 21, 01.11.1996, p. 6839-6852.

Research output: Contribution to journalArticle

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