Characterization of Clostridium difficile isolates from foals with diarrhea: 28 cases (1993-1997)

K G Magdesian, Dwight C. Hirsh, Spencer S. Jang, Lori M. Hansen, John E Madigan

Research output: Contribution to journalArticlepeer-review

66 Scopus citations


Objective - To determine molecular characteristics of Clostridium difficile isolates from foals with diarrhea and identify clinical abnormalities in affected foals. Design - Retrospective study. Animals - 28 foals with C difficile-associated diarrhea. Procedure - Toxigenicity, molecular fingerprinting, and antibiotic susceptibility patterns were determined. Information on signalment, clinical findings, results of clinicopathologic testing, whether antimicrobials had been administered prior to development of diarrhea, and outcome was obtained from the medical records. Results - Twenty-three (82%) foals survived. Toxin A and B gene sequences were detected in isolates from 24 of 27 foals, whereas the toxin B gene alone was detected in the isolate from 1 foal. Results of an ELISA for toxin A were positive for fecal samples from only 8 of 20 (40%) foals. Ten of 23 (43%) isolates were resistant to metronidazole. Molecular fingerprinting revealed marked heterogeneity among isolates, except for the metronidazole-resistant isolates. Sixteen foals had tachypnea. Hematologic abnormalities were indicative of inflammation. Common serum biochemical abnormalities included metabolic acidosis, hyponatremia, hypocalcemia, azotemia, hypoproteinemia, hyperglycemia, and high enzyme activities. Passive transfer of maternal antibodies was adequate in all 12 foals evaluated. Conclusions and Clinical Relevance - Results suggest that a large percentage of C difficile isolates from foals with diarrhea will have the toxin A and B gene sequences. Because of the possibility that isolates will be resistant to metronidazole, susceptibility testing is warranted. Clostridium difficile isolates from foals may have a substantial amount of molecular heterogeneity. Clinical and hematologic findings in affected foals are similar to those for foals with diarrhea caused by other pathogens.

Original languageEnglish (US)
Pages (from-to)67-73
Number of pages7
JournalJournal of the American Veterinary Medical Association
Issue number1
StatePublished - Jan 1 2002

ASJC Scopus subject areas

  • veterinary(all)


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