Characterization of cellular lipids in doxorubicin-sensitive and -resistant P388 mouse leukemia cells

Walter M. Holleran, Michael W. DeGregorio, Ram Ganapathi, Jordan R. Wilbur, Bruce A. Macher

Research output: Contribution to journalArticle

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Abstract

The purpose of this study was to determine whether changes in cellular lipid composition accompanied the selection of cells that are resistant to the anthracycline doxorubicin. Total cellular lipid extracts from doxorubicin-sensitive and doxorubicin-resistant P388 murine leukemia cells were prepared and separated into neutral glycosphingolipids, gangliosides, phospholipids, and neutral lipid families. No significant quantitative differences in total cholesterol, lipid-bound sialic acid, neutral hexose, and lipid-bound phosphate were found between the two cell lines. Gas-liquid chromatographic analysis of the fatty acids derived from each lipid class demonstrated that sensitive and resistant cells had essentially identical fatty acid compositions. Qualitative evaluation of the four lipid classes by high-performance thin layer chromatography revealed only minor differences in lipid composition between the resistant and the sensitive cells. Results from this study indicate that although minor differences between the two cell lines are present, no major cellular lipid differences are evident to account for the marked differences in the cellular pharmacokinetics and cytotoxic effects of doxorubicin between doxorubicin-sensitive and doxorubicin-resistant P388 murine leukemia a cells.

Original languageEnglish (US)
Pages (from-to)11-15
Number of pages5
JournalCancer Chemotherapy and Pharmacology
Volume17
Issue number1
DOIs
StatePublished - May 1986

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Leukemia P388
Doxorubicin
Lipids
Fatty Acids
Neutral Glycosphingolipids
Chemical analysis
Cells
Cell Line
Thin layer chromatography
Pharmacokinetics
Hexoses
Gangliosides
Anthracyclines
Liquid chromatography
N-Acetylneuraminic Acid
Thin Layer Chromatography
Gas Chromatography
Phospholipids
Gases
Phosphates

ASJC Scopus subject areas

  • Pharmacology
  • Oncology
  • Cancer Research

Cite this

Characterization of cellular lipids in doxorubicin-sensitive and -resistant P388 mouse leukemia cells. / Holleran, Walter M.; DeGregorio, Michael W.; Ganapathi, Ram; Wilbur, Jordan R.; Macher, Bruce A.

In: Cancer Chemotherapy and Pharmacology, Vol. 17, No. 1, 05.1986, p. 11-15.

Research output: Contribution to journalArticle

Holleran, Walter M. ; DeGregorio, Michael W. ; Ganapathi, Ram ; Wilbur, Jordan R. ; Macher, Bruce A. / Characterization of cellular lipids in doxorubicin-sensitive and -resistant P388 mouse leukemia cells. In: Cancer Chemotherapy and Pharmacology. 1986 ; Vol. 17, No. 1. pp. 11-15.
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