Characterization of cell lines developed from the glassy-winged sharpshooter, Homalodisca coagulata (Hemiptera: Cicadellidae)

Shizuo G. Kamita, Zung N. Do, Aman I. Samra, James R. Hagler, Bruce D. Hammock

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Four continuous cell lines were established from the embryos of the glassy-winged sharpshooter, Homalodisca coagulata (Say), an economically important insect vector of bacterial pathogens of grape, almond, citrus, oleander, and other agricultural and ornamental plantings. The cell lines were designated GWSS-Z10, GWSS-Z15, GWSS-G3, and GWSS-LH. The GWSS-Z10, GWSS-Z15, and GWSS-G3 lines were cultured in Ex-Cell 401 medium supplemented with 10% fetal bovine serum (FBS), whereas the GWSS-LH line was cultured in LH medium supplemented with 20% FBS. The cell lines were characterized in terms of their morphology, growth, protein composition, and polymerase chain reaction-amplification patterns of their chromosomal deoxyribonucleic acid. The population doubling times of GWSS-Z10, GWSS-Z15, GWSS-G3, and GWSS-LH were 46.2, 90.9, 100.3, and 60.2 h, respectively. These lines should be useful for the study of insect-pathogenic viruses of leafhoppers, aphids, treehoppers, and other related insects as well as plant-pathogenic viruses that are transmitted by these insects.

Original languageEnglish (US)
Pages (from-to)149-153
Number of pages5
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume41
Issue number5-6
DOIs
StatePublished - May 2005

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Hemiptera
Cells
Viruses
Cell Line
Insects
Nerium
Insect Viruses
Insect Vectors
Plant Viruses
Aphids
Citrus
Polymerase chain reaction
Vitis
Pathogens
Serum
Amplification
Embryonic Structures
Polymerase Chain Reaction
DNA
Growth

Keywords

  • GWSS
  • Insect
  • Pierce disease
  • Xylella fastidiosa

ASJC Scopus subject areas

  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Characterization of cell lines developed from the glassy-winged sharpshooter, Homalodisca coagulata (Hemiptera : Cicadellidae). / Kamita, Shizuo G.; Do, Zung N.; Samra, Aman I.; Hagler, James R.; Hammock, Bruce D.

In: In Vitro Cellular and Developmental Biology - Animal, Vol. 41, No. 5-6, 05.2005, p. 149-153.

Research output: Contribution to journalArticle

Kamita, Shizuo G. ; Do, Zung N. ; Samra, Aman I. ; Hagler, James R. ; Hammock, Bruce D. / Characterization of cell lines developed from the glassy-winged sharpshooter, Homalodisca coagulata (Hemiptera : Cicadellidae). In: In Vitro Cellular and Developmental Biology - Animal. 2005 ; Vol. 41, No. 5-6. pp. 149-153.
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