Abstract
Four continuous cell lines were established from the embryos of the glassy-winged sharpshooter, Homalodisca coagulata (Say), an economically important insect vector of bacterial pathogens of grape, almond, citrus, oleander, and other agricultural and ornamental plantings. The cell lines were designated GWSS-Z10, GWSS-Z15, GWSS-G3, and GWSS-LH. The GWSS-Z10, GWSS-Z15, and GWSS-G3 lines were cultured in Ex-Cell 401 medium supplemented with 10% fetal bovine serum (FBS), whereas the GWSS-LH line was cultured in LH medium supplemented with 20% FBS. The cell lines were characterized in terms of their morphology, growth, protein composition, and polymerase chain reaction-amplification patterns of their chromosomal deoxyribonucleic acid. The population doubling times of GWSS-Z10, GWSS-Z15, GWSS-G3, and GWSS-LH were 46.2, 90.9, 100.3, and 60.2 h, respectively. These lines should be useful for the study of insect-pathogenic viruses of leafhoppers, aphids, treehoppers, and other related insects as well as plant-pathogenic viruses that are transmitted by these insects.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 149-153 |
| Number of pages | 5 |
| Journal | In Vitro Cellular and Developmental Biology - Animal |
| Volume | 41 |
| Issue number | 5-6 |
| DOIs | |
| State | Published - May 2005 |
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Keywords
- GWSS
- Insect
- Pierce disease
- Xylella fastidiosa
ASJC Scopus subject areas
- Developmental Biology
- Clinical Biochemistry
- Cell Biology
Cite this
Characterization of cell lines developed from the glassy-winged sharpshooter, Homalodisca coagulata (Hemiptera : Cicadellidae). / Kamita, Shizuo G.; Do, Zung N.; Samra, Aman I.; Hagler, James R.; Hammock, Bruce D.
In: In Vitro Cellular and Developmental Biology - Animal, Vol. 41, No. 5-6, 05.2005, p. 149-153.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization of cell lines developed from the glassy-winged sharpshooter, Homalodisca coagulata (Hemiptera
T2 - Cicadellidae)
AU - Kamita, Shizuo G.
AU - Do, Zung N.
AU - Samra, Aman I.
AU - Hagler, James R.
AU - Hammock, Bruce D.
PY - 2005/5
Y1 - 2005/5
N2 - Four continuous cell lines were established from the embryos of the glassy-winged sharpshooter, Homalodisca coagulata (Say), an economically important insect vector of bacterial pathogens of grape, almond, citrus, oleander, and other agricultural and ornamental plantings. The cell lines were designated GWSS-Z10, GWSS-Z15, GWSS-G3, and GWSS-LH. The GWSS-Z10, GWSS-Z15, and GWSS-G3 lines were cultured in Ex-Cell 401 medium supplemented with 10% fetal bovine serum (FBS), whereas the GWSS-LH line was cultured in LH medium supplemented with 20% FBS. The cell lines were characterized in terms of their morphology, growth, protein composition, and polymerase chain reaction-amplification patterns of their chromosomal deoxyribonucleic acid. The population doubling times of GWSS-Z10, GWSS-Z15, GWSS-G3, and GWSS-LH were 46.2, 90.9, 100.3, and 60.2 h, respectively. These lines should be useful for the study of insect-pathogenic viruses of leafhoppers, aphids, treehoppers, and other related insects as well as plant-pathogenic viruses that are transmitted by these insects.
AB - Four continuous cell lines were established from the embryos of the glassy-winged sharpshooter, Homalodisca coagulata (Say), an economically important insect vector of bacterial pathogens of grape, almond, citrus, oleander, and other agricultural and ornamental plantings. The cell lines were designated GWSS-Z10, GWSS-Z15, GWSS-G3, and GWSS-LH. The GWSS-Z10, GWSS-Z15, and GWSS-G3 lines were cultured in Ex-Cell 401 medium supplemented with 10% fetal bovine serum (FBS), whereas the GWSS-LH line was cultured in LH medium supplemented with 20% FBS. The cell lines were characterized in terms of their morphology, growth, protein composition, and polymerase chain reaction-amplification patterns of their chromosomal deoxyribonucleic acid. The population doubling times of GWSS-Z10, GWSS-Z15, GWSS-G3, and GWSS-LH were 46.2, 90.9, 100.3, and 60.2 h, respectively. These lines should be useful for the study of insect-pathogenic viruses of leafhoppers, aphids, treehoppers, and other related insects as well as plant-pathogenic viruses that are transmitted by these insects.
KW - GWSS
KW - Insect
KW - Pierce disease
KW - Xylella fastidiosa
UR - http://www.scopus.com/inward/record.url?scp=24944487932&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=24944487932&partnerID=8YFLogxK
U2 - 10.1290/0501002.1
DO - 10.1290/0501002.1
M3 - Article
C2 - 16153147
AN - SCOPUS:24944487932
VL - 41
SP - 149
EP - 153
JO - In Vitro Cellular and Developmental Biology - Plant
JF - In Vitro Cellular and Developmental Biology - Plant
SN - 1054-5476
IS - 5-6
ER -