Characterization of buffalo-derived theilerial parasites with monoclonal antibodies and DNA probes.

Patricia A Conrad, O. K. ole-MoiYoi, C. L. Baldwin, T. T. Dolan, C. J. O'Callaghan, R. E. Njamunggeh, J. G. Grootenhuis, D. A. Stagg, B. L. Leitch, A. S. Young

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The characteristics of intra-lymphocytic Theileria isolated from African buffalo and from cattle that were infected with buffalo-derived parasites were evaluated using anti-schizont monoclonal antibodies (mAbs) and DNA probes. Antigenic differences were revealed by the reactivities of 27 mAbs with the buffalo-derived parasites isolated from different animals. Antigenic diversity was also seen with Theileria-infected lymphoblastoid cell isolates taken from the lymph nodes and lambda gt11, showed specific hybridization to parasite DNA in Southern blots of restriction enzyme-digested, lymphoblastoid cells infected with buffalo-derived theilerial parasites. Genotypic differences between the buffalo-derived parasites were revealed by the restriction fragment length polymorphisms seen with hybridization of those probes to DNA from cloned and uncloned Theileria-infected cell lines. The evaluation of theilerial parasites derived from buffalo and from cattle which underwent typical T. p. lawrencei reactions, after being infected with buffalo-derived theilerial parasites, did not show any specific phenotypic or genotypic characteristics of these parasites that would distinguish them from T. p. parva and T. p. bovis parasites. The validity of these subspecies distinctions is discussed.

Original languageEnglish (US)
Pages (from-to)179-188
Number of pages10
JournalParasitology
Volume98 Pt 2
StatePublished - Apr 1989

Fingerprint

Buffaloes
DNA probes
DNA Probes
buffaloes
monoclonal antibodies
Parasites
Monoclonal Antibodies
parasites
Theileria
Schizonts
Syncerus caffer
Antigenic Variation
antigenic variation
schizonts
cattle
DNA
Southern Blotting
Restriction Fragment Length Polymorphisms
Southern blotting
lymph nodes

ASJC Scopus subject areas

  • Immunology
  • Parasitology

Cite this

Conrad, P. A., ole-MoiYoi, O. K., Baldwin, C. L., Dolan, T. T., O'Callaghan, C. J., Njamunggeh, R. E., ... Young, A. S. (1989). Characterization of buffalo-derived theilerial parasites with monoclonal antibodies and DNA probes. Parasitology, 98 Pt 2, 179-188.

Characterization of buffalo-derived theilerial parasites with monoclonal antibodies and DNA probes. / Conrad, Patricia A; ole-MoiYoi, O. K.; Baldwin, C. L.; Dolan, T. T.; O'Callaghan, C. J.; Njamunggeh, R. E.; Grootenhuis, J. G.; Stagg, D. A.; Leitch, B. L.; Young, A. S.

In: Parasitology, Vol. 98 Pt 2, 04.1989, p. 179-188.

Research output: Contribution to journalArticle

Conrad, PA, ole-MoiYoi, OK, Baldwin, CL, Dolan, TT, O'Callaghan, CJ, Njamunggeh, RE, Grootenhuis, JG, Stagg, DA, Leitch, BL & Young, AS 1989, 'Characterization of buffalo-derived theilerial parasites with monoclonal antibodies and DNA probes.', Parasitology, vol. 98 Pt 2, pp. 179-188.
Conrad PA, ole-MoiYoi OK, Baldwin CL, Dolan TT, O'Callaghan CJ, Njamunggeh RE et al. Characterization of buffalo-derived theilerial parasites with monoclonal antibodies and DNA probes. Parasitology. 1989 Apr;98 Pt 2:179-188.
Conrad, Patricia A ; ole-MoiYoi, O. K. ; Baldwin, C. L. ; Dolan, T. T. ; O'Callaghan, C. J. ; Njamunggeh, R. E. ; Grootenhuis, J. G. ; Stagg, D. A. ; Leitch, B. L. ; Young, A. S. / Characterization of buffalo-derived theilerial parasites with monoclonal antibodies and DNA probes. In: Parasitology. 1989 ; Vol. 98 Pt 2. pp. 179-188.
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