Characterization of Anopheles pseudopunctipennis sensu lato from three countries of neotropical America from variation in allozymes and ribosomal DNA

J. G. Estrada-Franco, Gregory C Lanzaro, M. C. Ma, A. Walker-Abbey, P. Romans, C. Galvan- Sanchez, J. L. Cespedes, R. Vargas-Sagarnaga, A. Laughinghouse, I. Columbus, R. W. Gwadz

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Enzyme electrophoresis and restriction fragment length polymorphism (RFLP) analysis of Anopheles pseudopunctipennis sensu lato from nine isolated populations in neotropical America confirmed previous observations that it constitutes a species complex. Electrophoretic studies showed fixed differences at two enzyme loci, glycerol dehydrogenase (Gcd) and phosphoglucomutase (Pgm), suggesting limited or no gene flow between populations from Mexico and South America. In addition, analysis of genetic distance showed two distinctive clusters, one from Mexico and the other from South America, separated at a Nei's distance level of 0.13, a value consistent in magnitude with that of other anopheline sibling species. The RFLP analysis revealed the presence of a ribosomal DNA fragment in Mexican strains that was absent in strains from South America. Two species have been identified through these studies, one provisionally named An. pseudopunctipennis A, a species from central Mexico, and the other An. pseudopunctipennis B, for the species found in the interAndean valleys and Andean slopes in regions of Peru and Bolivia. This research provides information required to elucidate the status of the different species of the An. pseudopunctipennis complex as vectors of malaria in the Americas.

Original languageEnglish (US)
Pages (from-to)735-745
Number of pages11
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume49
Issue number6
StatePublished - 1993
Externally publishedYes

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

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    Estrada-Franco, J. G., Lanzaro, G. C., Ma, M. C., Walker-Abbey, A., Romans, P., Galvan- Sanchez, C., Cespedes, J. L., Vargas-Sagarnaga, R., Laughinghouse, A., Columbus, I., & Gwadz, R. W. (1993). Characterization of Anopheles pseudopunctipennis sensu lato from three countries of neotropical America from variation in allozymes and ribosomal DNA. American Journal of Tropical Medicine and Hygiene, 49(6), 735-745.