Characterization of Anaplasma phagocytophilum major surface protein 5 and the extent of its cross-reactivity with A. marginale

N. I. Strik, A. R. Alleman, A. F. Barbet, H. L. Sorenson, H. L. Wamsley, F. P. Gaschen, N. Luckschander, S. Wong, F. Chu, Janet E Foley, A. Bjoersdorff, S. Stuen, D. P. Knowles

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Major surface protein 5 (Msp5) of Anaplasma marginale is highly conserved in the genus Anaplasma and the antigen used in a commercially available competitive enzyme-linked immunosorbent assay (cELISA) for serologic identification of cattle with anaplasmosis. This study analyzes the degrees of conservation of Msp5 among various isolates of Anaplasma phagocytophilum and the extent of serologic cross-reactivity between recombinant Msp5 (rMsp5) of Anaplasma marginale and A. phagocytophilum. The msp5 genes from various isolates of A. phagocytophilum were sequenced and compared. rMsp5 proteins of A. phagocytophilum and A. marginale were used separately in an indirect ELISA to detect cross-reactivity in serum samples from humans and dogs infected with A. phagocytophilum and cattle infected with A marginale. Serum samples were also tested with a commercially available competitive ELISA that uses monoclonal antibody ANAF16C1. There were 100% sequence identities in the msp5 genes among all of the A. phagocytophilum isolates from the United States and a horse isolate from Sweden. Sheep isolates from Norway and dog isolates from Sweden were 99% identical to one another but differed in 17 base pairs from the United States isolates and the horse isolate. Serologic cross-reactivity was identified when serum samples from cattle infected with A. marginale were reacted with rMsp5 of A. phagocytophilum and when serum samples from humans and dogs infected with A. phagocytophilum were reacted with rMsp5 of A. marginale in an indirect-ELISA format. Serum samples from dogs or humans infected with A. phagocytophilum did not cross-react with rMsp5 of A. marginale when tested with the commercially available cELISA. These results suggest that rMsp5 of A. phagocytophilum is highly conserved among United States and European isolates and that serologic distinction between A. phagocytophilum and A. marginale infections cannot be accomplished if rMsp5 from either organism is used in an indirect ELISA.

Original languageEnglish (US)
Pages (from-to)262-268
Number of pages7
JournalClinical and Vaccine Immunology
Volume14
Issue number3
DOIs
StatePublished - Mar 2007

Fingerprint

Anaplasma phagocytophilum
Immunosorbents
Anaplasma marginale
Assays
Membrane Proteins
Genes
Staphylococcal Protein A
Enzymes
Recombinant Proteins
Enzyme-Linked Immunosorbent Assay
Conservation
Monoclonal Antibodies
Antigens
Dogs
Serum
Sweden
Horses
Anaplasma
Anaplasmosis
Anaplasma major surface protein 5

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Immunology
  • Immunology and Allergy
  • Microbiology (medical)

Cite this

Strik, N. I., Alleman, A. R., Barbet, A. F., Sorenson, H. L., Wamsley, H. L., Gaschen, F. P., ... Knowles, D. P. (2007). Characterization of Anaplasma phagocytophilum major surface protein 5 and the extent of its cross-reactivity with A. marginale. Clinical and Vaccine Immunology, 14(3), 262-268. https://doi.org/10.1128/CVI.00320-06

Characterization of Anaplasma phagocytophilum major surface protein 5 and the extent of its cross-reactivity with A. marginale. / Strik, N. I.; Alleman, A. R.; Barbet, A. F.; Sorenson, H. L.; Wamsley, H. L.; Gaschen, F. P.; Luckschander, N.; Wong, S.; Chu, F.; Foley, Janet E; Bjoersdorff, A.; Stuen, S.; Knowles, D. P.

In: Clinical and Vaccine Immunology, Vol. 14, No. 3, 03.2007, p. 262-268.

Research output: Contribution to journalArticle

Strik, NI, Alleman, AR, Barbet, AF, Sorenson, HL, Wamsley, HL, Gaschen, FP, Luckschander, N, Wong, S, Chu, F, Foley, JE, Bjoersdorff, A, Stuen, S & Knowles, DP 2007, 'Characterization of Anaplasma phagocytophilum major surface protein 5 and the extent of its cross-reactivity with A. marginale', Clinical and Vaccine Immunology, vol. 14, no. 3, pp. 262-268. https://doi.org/10.1128/CVI.00320-06
Strik, N. I. ; Alleman, A. R. ; Barbet, A. F. ; Sorenson, H. L. ; Wamsley, H. L. ; Gaschen, F. P. ; Luckschander, N. ; Wong, S. ; Chu, F. ; Foley, Janet E ; Bjoersdorff, A. ; Stuen, S. ; Knowles, D. P. / Characterization of Anaplasma phagocytophilum major surface protein 5 and the extent of its cross-reactivity with A. marginale. In: Clinical and Vaccine Immunology. 2007 ; Vol. 14, No. 3. pp. 262-268.
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AU - Wamsley, H. L.

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AU - Luckschander, N.

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