Abstract
The cleavage motif in the hemagglutinin (HA) protein of highly pathogenic H5 and H7 subtypes of avian influenza viruses is characterized by a peptide insertion or a multibasic cleavage site (MBCS). Here, we isolated an H4N2 virus from quails (Quail/CA12) with two additional arginines in the HA cleavage site, PEK. RRTR/G, forming an MBCS-like motif. Quail/CA12 is a reassortant virus with the HA and neuraminidase (NA) gene most similar to a duck-isolated H4N2 virus, PD/CA06 with a monobasic HA cleavage site. Quail/CA12 required exogenous trypsin for efficient growth in culture and caused no clinical illness in infected chickens. Quail/CA12 had high binding preference for α2,6-linked sialic acids and showed higher replication and transmission ability in chickens and quails than PD/CA06. Although the H4N2 virus remained low pathogenic, these data suggests that the acquisition of MBCS in the field is not restricted to H5 or H7 subtypes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 72-80 |
| Number of pages | 9 |
| Journal | Virology |
| Volume | 468-470 |
| DOIs | |
| State | Published - 2014 |
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Keywords
- H4N2
- Low pathogenic avian influenza
- Multibasic cleavage site
- Outbreak
- Quail
ASJC Scopus subject areas
- Virology
Cite this
Characterization of an H4N2 influenza virus from Quails with a multibasic motif in the hemagglutinin cleavage site. / Wong, Sook San; Yoon, Sun Woo; Zanin, Mark; Song, Min Suk; Oshansky, Christine; Zaraket, Hassan; Sonnberg, Stephanie; Rubrum, Adam; Seiler, Patrick; Ferguson, Angela; Krauss, Scott; Cardona, Carol; Webby, Richard J.; Crossley, Beate.
In: Virology, Vol. 468-470, 2014, p. 72-80.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization of an H4N2 influenza virus from Quails with a multibasic motif in the hemagglutinin cleavage site
AU - Wong, Sook San
AU - Yoon, Sun Woo
AU - Zanin, Mark
AU - Song, Min Suk
AU - Oshansky, Christine
AU - Zaraket, Hassan
AU - Sonnberg, Stephanie
AU - Rubrum, Adam
AU - Seiler, Patrick
AU - Ferguson, Angela
AU - Krauss, Scott
AU - Cardona, Carol
AU - Webby, Richard J.
AU - Crossley, Beate
PY - 2014
Y1 - 2014
N2 - The cleavage motif in the hemagglutinin (HA) protein of highly pathogenic H5 and H7 subtypes of avian influenza viruses is characterized by a peptide insertion or a multibasic cleavage site (MBCS). Here, we isolated an H4N2 virus from quails (Quail/CA12) with two additional arginines in the HA cleavage site, PEK. RRTR/G, forming an MBCS-like motif. Quail/CA12 is a reassortant virus with the HA and neuraminidase (NA) gene most similar to a duck-isolated H4N2 virus, PD/CA06 with a monobasic HA cleavage site. Quail/CA12 required exogenous trypsin for efficient growth in culture and caused no clinical illness in infected chickens. Quail/CA12 had high binding preference for α2,6-linked sialic acids and showed higher replication and transmission ability in chickens and quails than PD/CA06. Although the H4N2 virus remained low pathogenic, these data suggests that the acquisition of MBCS in the field is not restricted to H5 or H7 subtypes.
AB - The cleavage motif in the hemagglutinin (HA) protein of highly pathogenic H5 and H7 subtypes of avian influenza viruses is characterized by a peptide insertion or a multibasic cleavage site (MBCS). Here, we isolated an H4N2 virus from quails (Quail/CA12) with two additional arginines in the HA cleavage site, PEK. RRTR/G, forming an MBCS-like motif. Quail/CA12 is a reassortant virus with the HA and neuraminidase (NA) gene most similar to a duck-isolated H4N2 virus, PD/CA06 with a monobasic HA cleavage site. Quail/CA12 required exogenous trypsin for efficient growth in culture and caused no clinical illness in infected chickens. Quail/CA12 had high binding preference for α2,6-linked sialic acids and showed higher replication and transmission ability in chickens and quails than PD/CA06. Although the H4N2 virus remained low pathogenic, these data suggests that the acquisition of MBCS in the field is not restricted to H5 or H7 subtypes.
KW - H4N2
KW - Low pathogenic avian influenza
KW - Multibasic cleavage site
KW - Outbreak
KW - Quail
UR - http://www.scopus.com/inward/record.url?scp=84906482809&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84906482809&partnerID=8YFLogxK
U2 - 10.1016/j.virol.2014.07.048
DO - 10.1016/j.virol.2014.07.048
M3 - Article
VL - 468-470
SP - 72
EP - 80
JO - Virology
JF - Virology
SN - 0042-6822
ER -