Characterization of a second carotenoid β-hydroxylase gene from Arabidopsis and its relationship to the LUT1 locus

Li Tian, Dean Dellapenna

Research output: Contribution to journalArticle

72 Scopus citations

Abstract

Xanthophylls are oxygenated carotenoids that perform critical roles in plants. β-carotene hydroxylases (β-hydroxylases) add hydroxyl groups to the β-rings of carotenes and have been cloned from several bacteria and plants, including Arabidopsis. The lut1 mutation of Arabidopsis disrupts ε-ring hydroxylation and has been suggested to identify a related carotene hydroxylase that functions specifically on ε-ring structures. We have used library screening and genomics-based approaches to isolate a second β-hydroxylase genomic clone and its corresponding cDNA from Arabidopsis. The encoded protein is 70% identical to the previously reported Arabidopsis β-hydroxylase 1. Phylogenetic analysis indicates a common origin for the two proteins, however, their different chromosomal locations, intron positions and intron sizes suggest their duplication is not recent. Although both hydroxylases are expressed in all Arabidopsis tissues analyzed, β-hydroxylase 1 mRNA is always present at higher levels. Both cDNAs encode proteins that efficiently hydroxylate the C-3 position of β-ring containing carotenes and are only weakly active towards ε-ring containing carotenes. Neither β-hydroxylase cDNA maps to the LUT1 locus, and the genomic region encompassing the LUT1 locus does not contain a third related hydroxylase. These data indicate that the LUT1 locus encodes a protein necessary for ε-ring hydroxylation but unrelated to β-hydroxylases at the level of amino acid sequence.

Original languageEnglish (US)
Pages (from-to)379-388
Number of pages10
JournalPlant Molecular Biology
Volume47
Issue number3
DOIs
StatePublished - 2001
Externally publishedYes

Keywords

  • Arabidopsis
  • Carotenoid
  • Hydroxylase
  • lut1
  • TaqMan
  • Xanthophyll

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

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