Characterization of a phospholipase A2 in hamster lung and in vitro and in vivo effects of bleomycin on this enzyme

Q. Wang; S. N. Giri; D. M. Hyde

doi:10.1016/0952-3278(89)90023-9

Characterization of a phospholipase A₂ in hamster lung and in vitro and in vivo effects of bleomycin on this enzyme

Q. Wang, S. N. Giri, D. M. Hyde

Anatomy, Physiology and Cell Biology

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Phospholipase A₂ (PLA₂) activity from adult hamster lung was characterized using L-α-1-palmitoyl-2-arachidonyl-[arachidonyl-1-¹⁴C]-phosp hatidylcholine as the substrate. The released [¹⁴C]-arachidonic acid was separated by TLC. The enzyme activity increased with increasing incubation time (0-120 minutes), calcium ion concentration (0-25.0 mM) and protein (0-2.0 mg). The optimum pH was 8.0. Deoxycholate had a concentration dependent (0.1 to 0.5 mM) inhibitory effect on the activity. PLA₂ specific activity was the highest in mitochondrial fraction. PLA₂ activity following incubation with bleomycin was increased in a dose related fashion. In vivo study showed that both PLA₂ activity and collagen content in hamster lung were significantly elevated at 14 days followed intratracheal instillation of bleomycin. The activation of PLA₂ may play an important role in bleomycin-induced pulmonary toxicity.

Original language	English (US)
Pages (from-to)	85-92
Number of pages	8
Journal	Prostaglandins, Leukotrienes and Essential Fatty Acids
Volume	36
Issue number	2
DOIs	https://doi.org/10.1016/0952-3278(89)90023-9
State	Published - 1989

ASJC Scopus subject areas

Clinical Biochemistry
Endocrinology, Diabetes and Metabolism

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title = "Characterization of a phospholipase A2 in hamster lung and in vitro and in vivo effects of bleomycin on this enzyme",

abstract = "Phospholipase A2 (PLA2) activity from adult hamster lung was characterized using L-α-1-palmitoyl-2-arachidonyl-[arachidonyl-1-14C]-phosp hatidylcholine as the substrate. The released [14C]-arachidonic acid was separated by TLC. The enzyme activity increased with increasing incubation time (0-120 minutes), calcium ion concentration (0-25.0 mM) and protein (0-2.0 mg). The optimum pH was 8.0. Deoxycholate had a concentration dependent (0.1 to 0.5 mM) inhibitory effect on the activity. PLA2 specific activity was the highest in mitochondrial fraction. PLA2 activity following incubation with bleomycin was increased in a dose related fashion. In vivo study showed that both PLA2 activity and collagen content in hamster lung were significantly elevated at 14 days followed intratracheal instillation of bleomycin. The activation of PLA2 may play an important role in bleomycin-induced pulmonary toxicity.",

author = "Q. Wang and Giri, {S. N.} and Hyde, {D. M.}",

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T1 - Characterization of a phospholipase A2 in hamster lung and in vitro and in vivo effects of bleomycin on this enzyme

AU - Wang, Q.

AU - Giri, S. N.

AU - Hyde, D. M.

PY - 1989

Y1 - 1989

N2 - Phospholipase A2 (PLA2) activity from adult hamster lung was characterized using L-α-1-palmitoyl-2-arachidonyl-[arachidonyl-1-14C]-phosp hatidylcholine as the substrate. The released [14C]-arachidonic acid was separated by TLC. The enzyme activity increased with increasing incubation time (0-120 minutes), calcium ion concentration (0-25.0 mM) and protein (0-2.0 mg). The optimum pH was 8.0. Deoxycholate had a concentration dependent (0.1 to 0.5 mM) inhibitory effect on the activity. PLA2 specific activity was the highest in mitochondrial fraction. PLA2 activity following incubation with bleomycin was increased in a dose related fashion. In vivo study showed that both PLA2 activity and collagen content in hamster lung were significantly elevated at 14 days followed intratracheal instillation of bleomycin. The activation of PLA2 may play an important role in bleomycin-induced pulmonary toxicity.

AB - Phospholipase A2 (PLA2) activity from adult hamster lung was characterized using L-α-1-palmitoyl-2-arachidonyl-[arachidonyl-1-14C]-phosp hatidylcholine as the substrate. The released [14C]-arachidonic acid was separated by TLC. The enzyme activity increased with increasing incubation time (0-120 minutes), calcium ion concentration (0-25.0 mM) and protein (0-2.0 mg). The optimum pH was 8.0. Deoxycholate had a concentration dependent (0.1 to 0.5 mM) inhibitory effect on the activity. PLA2 specific activity was the highest in mitochondrial fraction. PLA2 activity following incubation with bleomycin was increased in a dose related fashion. In vivo study showed that both PLA2 activity and collagen content in hamster lung were significantly elevated at 14 days followed intratracheal instillation of bleomycin. The activation of PLA2 may play an important role in bleomycin-induced pulmonary toxicity.

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