Characterization of a novel bovine leukocyte protein involved in cell-cell adhesion

B. C. Taylor, J. Mattapallil, Peter F Moore, R. J. Scibienski, Jeffrey L Stott

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Preliminary characterization of an apparently novel bovine leukocyte adhesion protein is described. Two IgG1 monoclonal antibodies, UC-C1 and UC-H5, raised against established cultures of IL-2-dependent bovine peripheral blood lymphocytes (PBL) were found to react with an antigen expressed by the majority of bovine peripheral blood leukocytes. Immunoprecipitation and polyacrylamide gel electrophoresis of the antigen produced a distinct protein band of molecular weight 160,000, and additional diffuse protein bands of approximate molecular weight 180,000, 175,000 and 150,000. Two-color flow cytometric analyses showed that the antigen was expressed at low density on a small proportion of circulating B lymphocytes, but was highly expressed on all circulating T lymphocytes. The majority of monocytes and all granulocytes expressed the antigen at a density lower than that of T lymphocytes. Peripheral blood lymphocytes stimulated with concanavalin A had an approximately 3-fold increased expression of the antigen, which was apparent within 18 h and remained stable in long-term cultures. Expression of the antigen in thymus, analyzed by the immunoperoxidase technique, was predominantly restricted to thymocytes in the immediate subcapsular cortex and medulla; expression in lymph nodes and spleen was predominantly confined to lymphocytes in T-cell areas. Flow-cytometric analysis demonstrated that thymocytes and the majority of peripheral and mesenteric lymph node-derived T cells had relatively low surface density of antigen compared to circulating T cells. Binding of UC-C1 or UC-H5 to the antigen on lymphocytes induced homotypic aggregation. UC-C1 completely blocked binding of FITC-conjugated UC-H5 to blood mononuclear cells, suggesting that the antibodies recognize the same epitope or proximal epitopes.

Original languageEnglish (US)
Pages (from-to)252-260
Number of pages9
JournalTissue Antigens
Volume44
Issue number4
StatePublished - 1994

Fingerprint

Cell Adhesion
Leukocytes
Antigens
T-Lymphocytes
Proteins
Lymphocytes
Thymocytes
Epitopes
Molecular Weight
Lymph Nodes
Fluorescein-5-isothiocyanate
Surface Antigens
Concanavalin A
Immunoenzyme Techniques
Immunoprecipitation
Granulocytes
Thymus Gland
Interleukin-2
Polyacrylamide Gel Electrophoresis
Monocytes

Keywords

  • Adhesion molecules
  • Bovine
  • Leukocyte antigens

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Characterization of a novel bovine leukocyte protein involved in cell-cell adhesion. / Taylor, B. C.; Mattapallil, J.; Moore, Peter F; Scibienski, R. J.; Stott, Jeffrey L.

In: Tissue Antigens, Vol. 44, No. 4, 1994, p. 252-260.

Research output: Contribution to journalArticle

Taylor, BC, Mattapallil, J, Moore, PF, Scibienski, RJ & Stott, JL 1994, 'Characterization of a novel bovine leukocyte protein involved in cell-cell adhesion', Tissue Antigens, vol. 44, no. 4, pp. 252-260.
Taylor, B. C. ; Mattapallil, J. ; Moore, Peter F ; Scibienski, R. J. ; Stott, Jeffrey L. / Characterization of a novel bovine leukocyte protein involved in cell-cell adhesion. In: Tissue Antigens. 1994 ; Vol. 44, No. 4. pp. 252-260.
@article{5527d8354df243f580936ff56c54b0bb,
title = "Characterization of a novel bovine leukocyte protein involved in cell-cell adhesion",
abstract = "Preliminary characterization of an apparently novel bovine leukocyte adhesion protein is described. Two IgG1 monoclonal antibodies, UC-C1 and UC-H5, raised against established cultures of IL-2-dependent bovine peripheral blood lymphocytes (PBL) were found to react with an antigen expressed by the majority of bovine peripheral blood leukocytes. Immunoprecipitation and polyacrylamide gel electrophoresis of the antigen produced a distinct protein band of molecular weight 160,000, and additional diffuse protein bands of approximate molecular weight 180,000, 175,000 and 150,000. Two-color flow cytometric analyses showed that the antigen was expressed at low density on a small proportion of circulating B lymphocytes, but was highly expressed on all circulating T lymphocytes. The majority of monocytes and all granulocytes expressed the antigen at a density lower than that of T lymphocytes. Peripheral blood lymphocytes stimulated with concanavalin A had an approximately 3-fold increased expression of the antigen, which was apparent within 18 h and remained stable in long-term cultures. Expression of the antigen in thymus, analyzed by the immunoperoxidase technique, was predominantly restricted to thymocytes in the immediate subcapsular cortex and medulla; expression in lymph nodes and spleen was predominantly confined to lymphocytes in T-cell areas. Flow-cytometric analysis demonstrated that thymocytes and the majority of peripheral and mesenteric lymph node-derived T cells had relatively low surface density of antigen compared to circulating T cells. Binding of UC-C1 or UC-H5 to the antigen on lymphocytes induced homotypic aggregation. UC-C1 completely blocked binding of FITC-conjugated UC-H5 to blood mononuclear cells, suggesting that the antibodies recognize the same epitope or proximal epitopes.",
keywords = "Adhesion molecules, Bovine, Leukocyte antigens",
author = "Taylor, {B. C.} and J. Mattapallil and Moore, {Peter F} and Scibienski, {R. J.} and Stott, {Jeffrey L}",
year = "1994",
language = "English (US)",
volume = "44",
pages = "252--260",
journal = "HLA",
issn = "2059-2302",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Characterization of a novel bovine leukocyte protein involved in cell-cell adhesion

AU - Taylor, B. C.

AU - Mattapallil, J.

AU - Moore, Peter F

AU - Scibienski, R. J.

AU - Stott, Jeffrey L

PY - 1994

Y1 - 1994

N2 - Preliminary characterization of an apparently novel bovine leukocyte adhesion protein is described. Two IgG1 monoclonal antibodies, UC-C1 and UC-H5, raised against established cultures of IL-2-dependent bovine peripheral blood lymphocytes (PBL) were found to react with an antigen expressed by the majority of bovine peripheral blood leukocytes. Immunoprecipitation and polyacrylamide gel electrophoresis of the antigen produced a distinct protein band of molecular weight 160,000, and additional diffuse protein bands of approximate molecular weight 180,000, 175,000 and 150,000. Two-color flow cytometric analyses showed that the antigen was expressed at low density on a small proportion of circulating B lymphocytes, but was highly expressed on all circulating T lymphocytes. The majority of monocytes and all granulocytes expressed the antigen at a density lower than that of T lymphocytes. Peripheral blood lymphocytes stimulated with concanavalin A had an approximately 3-fold increased expression of the antigen, which was apparent within 18 h and remained stable in long-term cultures. Expression of the antigen in thymus, analyzed by the immunoperoxidase technique, was predominantly restricted to thymocytes in the immediate subcapsular cortex and medulla; expression in lymph nodes and spleen was predominantly confined to lymphocytes in T-cell areas. Flow-cytometric analysis demonstrated that thymocytes and the majority of peripheral and mesenteric lymph node-derived T cells had relatively low surface density of antigen compared to circulating T cells. Binding of UC-C1 or UC-H5 to the antigen on lymphocytes induced homotypic aggregation. UC-C1 completely blocked binding of FITC-conjugated UC-H5 to blood mononuclear cells, suggesting that the antibodies recognize the same epitope or proximal epitopes.

AB - Preliminary characterization of an apparently novel bovine leukocyte adhesion protein is described. Two IgG1 monoclonal antibodies, UC-C1 and UC-H5, raised against established cultures of IL-2-dependent bovine peripheral blood lymphocytes (PBL) were found to react with an antigen expressed by the majority of bovine peripheral blood leukocytes. Immunoprecipitation and polyacrylamide gel electrophoresis of the antigen produced a distinct protein band of molecular weight 160,000, and additional diffuse protein bands of approximate molecular weight 180,000, 175,000 and 150,000. Two-color flow cytometric analyses showed that the antigen was expressed at low density on a small proportion of circulating B lymphocytes, but was highly expressed on all circulating T lymphocytes. The majority of monocytes and all granulocytes expressed the antigen at a density lower than that of T lymphocytes. Peripheral blood lymphocytes stimulated with concanavalin A had an approximately 3-fold increased expression of the antigen, which was apparent within 18 h and remained stable in long-term cultures. Expression of the antigen in thymus, analyzed by the immunoperoxidase technique, was predominantly restricted to thymocytes in the immediate subcapsular cortex and medulla; expression in lymph nodes and spleen was predominantly confined to lymphocytes in T-cell areas. Flow-cytometric analysis demonstrated that thymocytes and the majority of peripheral and mesenteric lymph node-derived T cells had relatively low surface density of antigen compared to circulating T cells. Binding of UC-C1 or UC-H5 to the antigen on lymphocytes induced homotypic aggregation. UC-C1 completely blocked binding of FITC-conjugated UC-H5 to blood mononuclear cells, suggesting that the antibodies recognize the same epitope or proximal epitopes.

KW - Adhesion molecules

KW - Bovine

KW - Leukocyte antigens

UR - http://www.scopus.com/inward/record.url?scp=0028033377&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028033377&partnerID=8YFLogxK

M3 - Article

C2 - 7532875

AN - SCOPUS:0028033377

VL - 44

SP - 252

EP - 260

JO - HLA

JF - HLA

SN - 2059-2302

IS - 4

ER -