Characterization of a novel alloherpesvirus from Atlantic cod (Gadus morhua)

Mar Marcos-Lopez, Thomas B. Waltzek, Ronald Hedrick, Dolores Baxa, Amber F. Garber, Rebecca Liston, Eric Johnsen, Benjamin S. Forward, Steven Backman, Hugh W. Ferguson

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Alloherpesviruses affect freshwater and marine fish species. The aim of the current study was to characterize a novel alloherpesvirus in Atlantic cod (Gadus morhua). Samples were processed for histopathology, transmission electron microscopy (TEM), virus isolation, molecular characterization, and in situ hybridization (ISH). Histopathology revealed that the infection was restricted to the gills and that it induced cytomegaly in infected cells. By TEM, numerous viral particles with morphology compatible with a herpesvirus were observed inside the cytomegalic cells. To characterize this new agent, polymerase chain reaction amplified regions of the ATPase subunit of the terminase, and DNA polymerase genes were sequenced. Phylogenetic analysis revealed strongest similarity with alloherpesviruses belonging to the genus Ictalurivirus and Salmonivirus. The ISH showed specific labeling of nuclear inclusions in the cytomegalic cells. While virus isolation was unsuccessful, the results obtained through different diagnostic tests in the present study confirm the discovery of a new alloherpesvirus affecting Atlantic cod. The authors propose the formal species designation Gadid herpesvirus 1 (GaHV-1) to be considered for approval by the International Committee on the Taxonomy of Viruses.

Original languageEnglish (US)
Pages (from-to)65-73
Number of pages9
JournalJournal of Veterinary Diagnostic Investigation
Volume24
Issue number1
DOIs
StatePublished - Jan 1 2012

Fingerprint

Gadus morhua
Herpesviridae
Ictalurivirus
Salmonivirus
Viruses
Transmission Electron Microscopy
histopathology
viruses
in situ hybridization
In Situ Hybridization
transmission electron microscopy
nuclear inclusions
Intranuclear Inclusion Bodies
DNA-directed DNA polymerase
cells
DNA-Directed DNA Polymerase
Fresh Water
virion
Routine Diagnostic Tests
freshwater fish

Keywords

  • Alloherpesvirus
  • Atlantic cod
  • in situ hybridization
  • polymerase chain reaction

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Characterization of a novel alloherpesvirus from Atlantic cod (Gadus morhua). / Marcos-Lopez, Mar; Waltzek, Thomas B.; Hedrick, Ronald; Baxa, Dolores; Garber, Amber F.; Liston, Rebecca; Johnsen, Eric; Forward, Benjamin S.; Backman, Steven; Ferguson, Hugh W.

In: Journal of Veterinary Diagnostic Investigation, Vol. 24, No. 1, 01.01.2012, p. 65-73.

Research output: Contribution to journalArticle

Marcos-Lopez, M, Waltzek, TB, Hedrick, R, Baxa, D, Garber, AF, Liston, R, Johnsen, E, Forward, BS, Backman, S & Ferguson, HW 2012, 'Characterization of a novel alloherpesvirus from Atlantic cod (Gadus morhua)', Journal of Veterinary Diagnostic Investigation, vol. 24, no. 1, pp. 65-73. https://doi.org/10.1177/1040638711416629
Marcos-Lopez, Mar ; Waltzek, Thomas B. ; Hedrick, Ronald ; Baxa, Dolores ; Garber, Amber F. ; Liston, Rebecca ; Johnsen, Eric ; Forward, Benjamin S. ; Backman, Steven ; Ferguson, Hugh W. / Characterization of a novel alloherpesvirus from Atlantic cod (Gadus morhua). In: Journal of Veterinary Diagnostic Investigation. 2012 ; Vol. 24, No. 1. pp. 65-73.
@article{949bbf6127ec4aa4ae9287bb1fb7eb73,
title = "Characterization of a novel alloherpesvirus from Atlantic cod (Gadus morhua)",
abstract = "Alloherpesviruses affect freshwater and marine fish species. The aim of the current study was to characterize a novel alloherpesvirus in Atlantic cod (Gadus morhua). Samples were processed for histopathology, transmission electron microscopy (TEM), virus isolation, molecular characterization, and in situ hybridization (ISH). Histopathology revealed that the infection was restricted to the gills and that it induced cytomegaly in infected cells. By TEM, numerous viral particles with morphology compatible with a herpesvirus were observed inside the cytomegalic cells. To characterize this new agent, polymerase chain reaction amplified regions of the ATPase subunit of the terminase, and DNA polymerase genes were sequenced. Phylogenetic analysis revealed strongest similarity with alloherpesviruses belonging to the genus Ictalurivirus and Salmonivirus. The ISH showed specific labeling of nuclear inclusions in the cytomegalic cells. While virus isolation was unsuccessful, the results obtained through different diagnostic tests in the present study confirm the discovery of a new alloherpesvirus affecting Atlantic cod. The authors propose the formal species designation Gadid herpesvirus 1 (GaHV-1) to be considered for approval by the International Committee on the Taxonomy of Viruses.",
keywords = "Alloherpesvirus, Atlantic cod, in situ hybridization, polymerase chain reaction",
author = "Mar Marcos-Lopez and Waltzek, {Thomas B.} and Ronald Hedrick and Dolores Baxa and Garber, {Amber F.} and Rebecca Liston and Eric Johnsen and Forward, {Benjamin S.} and Steven Backman and Ferguson, {Hugh W.}",
year = "2012",
month = "1",
day = "1",
doi = "10.1177/1040638711416629",
language = "English (US)",
volume = "24",
pages = "65--73",
journal = "Journal of Veterinary Diagnostic Investigation",
issn = "1040-6387",
publisher = "American Association of Veterinary Laboratory Diagnosticians",
number = "1",

}

TY - JOUR

T1 - Characterization of a novel alloherpesvirus from Atlantic cod (Gadus morhua)

AU - Marcos-Lopez, Mar

AU - Waltzek, Thomas B.

AU - Hedrick, Ronald

AU - Baxa, Dolores

AU - Garber, Amber F.

AU - Liston, Rebecca

AU - Johnsen, Eric

AU - Forward, Benjamin S.

AU - Backman, Steven

AU - Ferguson, Hugh W.

PY - 2012/1/1

Y1 - 2012/1/1

N2 - Alloherpesviruses affect freshwater and marine fish species. The aim of the current study was to characterize a novel alloherpesvirus in Atlantic cod (Gadus morhua). Samples were processed for histopathology, transmission electron microscopy (TEM), virus isolation, molecular characterization, and in situ hybridization (ISH). Histopathology revealed that the infection was restricted to the gills and that it induced cytomegaly in infected cells. By TEM, numerous viral particles with morphology compatible with a herpesvirus were observed inside the cytomegalic cells. To characterize this new agent, polymerase chain reaction amplified regions of the ATPase subunit of the terminase, and DNA polymerase genes were sequenced. Phylogenetic analysis revealed strongest similarity with alloherpesviruses belonging to the genus Ictalurivirus and Salmonivirus. The ISH showed specific labeling of nuclear inclusions in the cytomegalic cells. While virus isolation was unsuccessful, the results obtained through different diagnostic tests in the present study confirm the discovery of a new alloherpesvirus affecting Atlantic cod. The authors propose the formal species designation Gadid herpesvirus 1 (GaHV-1) to be considered for approval by the International Committee on the Taxonomy of Viruses.

AB - Alloherpesviruses affect freshwater and marine fish species. The aim of the current study was to characterize a novel alloherpesvirus in Atlantic cod (Gadus morhua). Samples were processed for histopathology, transmission electron microscopy (TEM), virus isolation, molecular characterization, and in situ hybridization (ISH). Histopathology revealed that the infection was restricted to the gills and that it induced cytomegaly in infected cells. By TEM, numerous viral particles with morphology compatible with a herpesvirus were observed inside the cytomegalic cells. To characterize this new agent, polymerase chain reaction amplified regions of the ATPase subunit of the terminase, and DNA polymerase genes were sequenced. Phylogenetic analysis revealed strongest similarity with alloherpesviruses belonging to the genus Ictalurivirus and Salmonivirus. The ISH showed specific labeling of nuclear inclusions in the cytomegalic cells. While virus isolation was unsuccessful, the results obtained through different diagnostic tests in the present study confirm the discovery of a new alloherpesvirus affecting Atlantic cod. The authors propose the formal species designation Gadid herpesvirus 1 (GaHV-1) to be considered for approval by the International Committee on the Taxonomy of Viruses.

KW - Alloherpesvirus

KW - Atlantic cod

KW - in situ hybridization

KW - polymerase chain reaction

UR - http://www.scopus.com/inward/record.url?scp=84857321483&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84857321483&partnerID=8YFLogxK

U2 - 10.1177/1040638711416629

DO - 10.1177/1040638711416629

M3 - Article

C2 - 22362936

AN - SCOPUS:84857321483

VL - 24

SP - 65

EP - 73

JO - Journal of Veterinary Diagnostic Investigation

JF - Journal of Veterinary Diagnostic Investigation

SN - 1040-6387

IS - 1

ER -