Characterization of a HTLV-I-infected cell line derived from a patient with adult T-cell leukemia with stable co-expression of CD4 and CD8

Thomas Rowe, Charlene Dezzutti, Patricia C. Guenthner, Lee Lam, Thomas Hodge, Michael Dale Lairmore, Renu B. Lal, Thomas M. Folks

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

A long-term T-cell line, termed SP+, was developed from a human T-cell leukemia virus type I (HTLV-I)-infected patient with adult T-cell leukemia that is dependent on exogenous IL-2 for growth. The SP+ expresses a full complimentation of HTLV-I-specifrc viral proteins, and contains replication competent viral particles. Restriction enzyme digestion followed by Southern blot analysis demonstrated the presence of a single integrated proviral copy and limiting dilution analysis confirmed the clonality of the cell line. Interestingly, phenotypically, the SP+ cell line is CD2+, CD3+ and coexpresses CD4 and CD8, yet lacks TCRαβ and TCRτδ expression. Further ontogenetic characterization of the SP+ cell line demonstrated the lack of thymic T-cell precursor markers, including absence of cell surface expression of CD1, intracellular thymic terminal deoxynucleotidyl transferase (TdT) enzyme, as well as message expression for V(D)J recombinase activating gene-1 (RAG-1). Furthermore, the SP+ cell did express the message for the CD3δ chain. Taken together, these data suggest that the SP+ cell line resulted from HTLV-I infection of a mature CD4+/CDB+ lymphocyte. This cell line can be potentially useful as a model, both for regulation of cellular functions by HTLV-I and for immunologic functions of mature dual CD4/CD8 positive T-cells.

Original languageEnglish (US)
Pages (from-to)621-628
Number of pages8
JournalLeukemia Research
Volume19
Issue number9
DOIs
StatePublished - 1995
Externally publishedYes

Keywords

  • ATL
  • cell line
  • dual CD CD8 positive

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

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