Characterization of a degenerative cardiomyopathy associated with domoic acid toxicity in california sea lions (Zalophus californianus)

T. S. Zabka, Tracey Goldstein, C. Cross, R. W. Mueller, Christine K Johnson, S. Gill, F. M D Gulland

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Domoic acid, produced by marine algae, can cause acute and chronic neurologic sequela in California sea lions (Zalophus califomianus) from acute toxicity or sublethal exposure. Eight sea lions, representing acute and chronic cases, both sexes, and all age classes, were selected to demonstrate a concurrent degenerative cardiomyopathy. Critical aspects of characterizing the cardiomyopathy by lesion distribution and morphology were the development of a heart dissection and tissue-trimming protocol and the delineation of the cardiac conducting system by histomorphology and immunohis- tochemistry for neuron-specific protein gene product 9.5. Histopathologic features and progression of the cardiomyopathy are described, varying from acute to chronic active and mild to severe. The cardiomyopathy is distinguished from other heart lesions in pinnipeds. Based on histopathologic features, immunopositive staining for cleaved caspase-3, and comparison with known, similar-appearing cardiomyopathies, the proposed pathogenesis for the degenerative cardiomyopathy is the primary or at least initial direct interaction of domoic acid with receptors that are suspected to exist in the heart, l- Carnitine, measured in the heart and skeletal muscle, and troponin-I, measured in serum collected at the time of death from additional animals (n = 58), were not predictive of the domoic acid-associated cardiomyopathy. This degenerative cardiomyopathy in California sea lions represents another syndrome beyond central neurologic disease associated with exposure to domoic acid and may contribute to morbidity and mortality.

Original languageEnglish (US)
Pages (from-to)105-119
Number of pages15
JournalVeterinary Pathology
Volume46
Issue number1
DOIs
StatePublished - Jan 2009

Fingerprint

Sea Lions
domoic acid
cardiomyopathy
Cardiomyopathies
toxicity
heart
algae
lesions (animal)
Pinnipedia
Zalophus
central nervous system diseases
Troponin I
Zalophus californianus
Carnitine
Otariidae
carnitine
complications (disease)
Central Nervous System Diseases
caspase-3
acute toxicity

Keywords

  • Algal blooms
  • California sea lions
  • Carnitine, caspase
  • Conducting system
  • Heart
  • Immunohistochemistry
  • Troponin-I

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Characterization of a degenerative cardiomyopathy associated with domoic acid toxicity in california sea lions (Zalophus californianus). / Zabka, T. S.; Goldstein, Tracey; Cross, C.; Mueller, R. W.; Johnson, Christine K; Gill, S.; Gulland, F. M D.

In: Veterinary Pathology, Vol. 46, No. 1, 01.2009, p. 105-119.

Research output: Contribution to journalArticle

@article{9b0d342e85c24bf49374b705bae86a19,
title = "Characterization of a degenerative cardiomyopathy associated with domoic acid toxicity in california sea lions (Zalophus californianus)",
abstract = "Domoic acid, produced by marine algae, can cause acute and chronic neurologic sequela in California sea lions (Zalophus califomianus) from acute toxicity or sublethal exposure. Eight sea lions, representing acute and chronic cases, both sexes, and all age classes, were selected to demonstrate a concurrent degenerative cardiomyopathy. Critical aspects of characterizing the cardiomyopathy by lesion distribution and morphology were the development of a heart dissection and tissue-trimming protocol and the delineation of the cardiac conducting system by histomorphology and immunohis- tochemistry for neuron-specific protein gene product 9.5. Histopathologic features and progression of the cardiomyopathy are described, varying from acute to chronic active and mild to severe. The cardiomyopathy is distinguished from other heart lesions in pinnipeds. Based on histopathologic features, immunopositive staining for cleaved caspase-3, and comparison with known, similar-appearing cardiomyopathies, the proposed pathogenesis for the degenerative cardiomyopathy is the primary or at least initial direct interaction of domoic acid with receptors that are suspected to exist in the heart, l- Carnitine, measured in the heart and skeletal muscle, and troponin-I, measured in serum collected at the time of death from additional animals (n = 58), were not predictive of the domoic acid-associated cardiomyopathy. This degenerative cardiomyopathy in California sea lions represents another syndrome beyond central neurologic disease associated with exposure to domoic acid and may contribute to morbidity and mortality.",
keywords = "Algal blooms, California sea lions, Carnitine, caspase, Conducting system, Heart, Immunohistochemistry, Troponin-I",
author = "Zabka, {T. S.} and Tracey Goldstein and C. Cross and Mueller, {R. W.} and Johnson, {Christine K} and S. Gill and Gulland, {F. M D}",
year = "2009",
month = "1",
doi = "10.1354/vp.46-1-105",
language = "English (US)",
volume = "46",
pages = "105--119",
journal = "Veterinary Pathology",
issn = "0300-9858",
publisher = "SAGE Publications Ltd",
number = "1",

}

TY - JOUR

T1 - Characterization of a degenerative cardiomyopathy associated with domoic acid toxicity in california sea lions (Zalophus californianus)

AU - Zabka, T. S.

AU - Goldstein, Tracey

AU - Cross, C.

AU - Mueller, R. W.

AU - Johnson, Christine K

AU - Gill, S.

AU - Gulland, F. M D

PY - 2009/1

Y1 - 2009/1

N2 - Domoic acid, produced by marine algae, can cause acute and chronic neurologic sequela in California sea lions (Zalophus califomianus) from acute toxicity or sublethal exposure. Eight sea lions, representing acute and chronic cases, both sexes, and all age classes, were selected to demonstrate a concurrent degenerative cardiomyopathy. Critical aspects of characterizing the cardiomyopathy by lesion distribution and morphology were the development of a heart dissection and tissue-trimming protocol and the delineation of the cardiac conducting system by histomorphology and immunohis- tochemistry for neuron-specific protein gene product 9.5. Histopathologic features and progression of the cardiomyopathy are described, varying from acute to chronic active and mild to severe. The cardiomyopathy is distinguished from other heart lesions in pinnipeds. Based on histopathologic features, immunopositive staining for cleaved caspase-3, and comparison with known, similar-appearing cardiomyopathies, the proposed pathogenesis for the degenerative cardiomyopathy is the primary or at least initial direct interaction of domoic acid with receptors that are suspected to exist in the heart, l- Carnitine, measured in the heart and skeletal muscle, and troponin-I, measured in serum collected at the time of death from additional animals (n = 58), were not predictive of the domoic acid-associated cardiomyopathy. This degenerative cardiomyopathy in California sea lions represents another syndrome beyond central neurologic disease associated with exposure to domoic acid and may contribute to morbidity and mortality.

AB - Domoic acid, produced by marine algae, can cause acute and chronic neurologic sequela in California sea lions (Zalophus califomianus) from acute toxicity or sublethal exposure. Eight sea lions, representing acute and chronic cases, both sexes, and all age classes, were selected to demonstrate a concurrent degenerative cardiomyopathy. Critical aspects of characterizing the cardiomyopathy by lesion distribution and morphology were the development of a heart dissection and tissue-trimming protocol and the delineation of the cardiac conducting system by histomorphology and immunohis- tochemistry for neuron-specific protein gene product 9.5. Histopathologic features and progression of the cardiomyopathy are described, varying from acute to chronic active and mild to severe. The cardiomyopathy is distinguished from other heart lesions in pinnipeds. Based on histopathologic features, immunopositive staining for cleaved caspase-3, and comparison with known, similar-appearing cardiomyopathies, the proposed pathogenesis for the degenerative cardiomyopathy is the primary or at least initial direct interaction of domoic acid with receptors that are suspected to exist in the heart, l- Carnitine, measured in the heart and skeletal muscle, and troponin-I, measured in serum collected at the time of death from additional animals (n = 58), were not predictive of the domoic acid-associated cardiomyopathy. This degenerative cardiomyopathy in California sea lions represents another syndrome beyond central neurologic disease associated with exposure to domoic acid and may contribute to morbidity and mortality.

KW - Algal blooms

KW - California sea lions

KW - Carnitine, caspase

KW - Conducting system

KW - Heart

KW - Immunohistochemistry

KW - Troponin-I

UR - http://www.scopus.com/inward/record.url?scp=63249134755&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=63249134755&partnerID=8YFLogxK

U2 - 10.1354/vp.46-1-105

DO - 10.1354/vp.46-1-105

M3 - Article

C2 - 19112124

AN - SCOPUS:63249134755

VL - 46

SP - 105

EP - 119

JO - Veterinary Pathology

JF - Veterinary Pathology

SN - 0300-9858

IS - 1

ER -