Characterization of a continuous feline mammary epithelial cell line susceptible to feline epitheliotropic viruses

Patricia Pesavento, Hongwei Liu, Robert J. Ossiboff, Karla M. Stucker, Anna Heymer, Lee Millon, Jason Wood, Deborah van der List, John S L Parker

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Mucosal epithelial cells are the primary targets for many common viral pathogens of cats. Viral infection of epithelia can damage or disrupt the epithelial barrier that protects underlying tissues. In vitro cell culture systems are an effective means to study how viruses infect and disrupt epithelial barriers, however no true continuous or immortalized feline epithelial cell culture lines are available. A continuous cell culture of feline mammary epithelial cells (FMEC UCD-04-2) that forms tight junctions with high transepithelial electrical resistance (>2000 Ω cm-1) 3-4 days after reaching confluence was characterized. In addition, it was shown that FMECs are susceptible to infection with feline calicivirus (FCV), feline herpesvirus (FHV-1), feline coronavirus (FeCoV), and feline panleukopenia virus (FPV). These cells will be useful for studies of feline viral disease and for in vitro studies of feline epithelia.

Original languageEnglish (US)
Pages (from-to)105-110
Number of pages6
JournalJournal of Virological Methods
Volume157
Issue number1
DOIs
StatePublished - Apr 2009

Fingerprint

Felidae
Breast
Epithelial Cells
Viruses
Cell Line
Cell Culture Techniques
Virus Diseases
Feline Panleukopenia Virus
Feline Coronavirus
Epithelium
Feline Calicivirus
Cat Diseases
Tight Junctions
Herpesviridae
Electric Impedance
Cats
Infection
In Vitro Techniques

Keywords

  • Cell line
  • Epithelial
  • Feline
  • Junctional adhesion molecule-A
  • Tight junction
  • Virus susceptibility

ASJC Scopus subject areas

  • Virology

Cite this

Characterization of a continuous feline mammary epithelial cell line susceptible to feline epitheliotropic viruses. / Pesavento, Patricia; Liu, Hongwei; Ossiboff, Robert J.; Stucker, Karla M.; Heymer, Anna; Millon, Lee; Wood, Jason; van der List, Deborah; Parker, John S L.

In: Journal of Virological Methods, Vol. 157, No. 1, 04.2009, p. 105-110.

Research output: Contribution to journalArticle

Pesavento, P, Liu, H, Ossiboff, RJ, Stucker, KM, Heymer, A, Millon, L, Wood, J, van der List, D & Parker, JSL 2009, 'Characterization of a continuous feline mammary epithelial cell line susceptible to feline epitheliotropic viruses', Journal of Virological Methods, vol. 157, no. 1, pp. 105-110. https://doi.org/10.1016/j.jviromet.2008.11.018
Pesavento, Patricia ; Liu, Hongwei ; Ossiboff, Robert J. ; Stucker, Karla M. ; Heymer, Anna ; Millon, Lee ; Wood, Jason ; van der List, Deborah ; Parker, John S L. / Characterization of a continuous feline mammary epithelial cell line susceptible to feline epitheliotropic viruses. In: Journal of Virological Methods. 2009 ; Vol. 157, No. 1. pp. 105-110.
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