Characterization of a cashew allergen, 11S globulin (Ana o 2), conformational epitope

Jason M. Robotham, Lixin Xia, LeAnna N. Willison, Suzanne S Teuber, Shridhar K. Sathe, Kenneth H. Roux

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Both linear and conformational epitopes likely contribute to the allergenicity of tree nut allergens, yet, due largely to technical issues, few conformational epitopes have been characterized. Using the well studied recombinant cashew allergen, Ana o 2, an 11S globulin or legumin, we identified a murine monoclonal antibody which recognizes a conformational epitope and competes with patient IgE Ana o 2-reactive antibodies. This epitope is expressed on the large subunit of Ana o 2, but only when associated with an 11S globulin small subunit. Both Ana o 2 and the homologous soybean Gly m 6 small subunits can foster epitope expression, even when the natural N-terminal to C-terminal subunit order is reversed in chimeric molecules. The epitope, which is also expressed on native Ana o 2, is readily susceptible to destruction by physical and chemical denaturants.

Original languageEnglish (US)
Pages (from-to)1830-1838
Number of pages9
JournalMolecular Immunology
Volume47
Issue number9
DOIs
StatePublished - May 2010

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Keywords

  • 11S Globulin
  • Cashew
  • Conformational epitope
  • Food allergy
  • Legumin
  • Tree nut

ASJC Scopus subject areas

  • Molecular Biology
  • Immunology

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