Abstract
The immune response elicited by a synthetic peptide derived from an immunodominant external envelope region (Env-5, amino acids 242-257) of human T-lymphotropic virus type 1 (HTLV-I) was tested in a rabbit model of HTLV-I infection. The synthetic peptide elicited a strong antibody response to the HTLV-I envelope protein gp46; however, these antibodies failed to inhibit HTLV-I-mediated cell fusion. Immunized rabbits were not protected from HTLV-I infection as determined by seroconversion to viral core proteins by immunoblot, HTLV-I p24 antigen detection in lymphocyte cultures and polymerase chain reaction for the HTLV-I provirus in lymphocyte DNA. Env-5 peptide immunization failed to induce T-cell lymphocyte proliferative responses in rabbits, but induced antibody responses in T-cell deficient Balb c nu/nu mice suggesting that the antigenic determinant represented by the Env-5 peptide is primarily a B-cell epitope. These results further define an immunodominant epitope of the HTLV-I envelope protein and suggest that potential synthetic peptide vaccines against HTLV-I infection must contain multiple antigens that induce both humoral and cellular immune reactivity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 11-20 |
| Number of pages | 10 |
| Journal | Cancer Letters |
| Volume | 66 |
| Issue number | 1 |
| DOIs | |
| State | Published - Sep 14 1992 |
| Externally published | Yes |
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Keywords
- animal model
- human
- human T-lymphotropic virus type 1
- synthetic peptide
- vaccine
ASJC Scopus subject areas
- Cancer Research
- Molecular Biology
- Oncology
Cite this
Characterization of a B-cell immunodominant epitope of human T-lymphotropic virus type 1 (HTLV-I) envelope gp46. / Lairmore, Michael Dale; Rudolph, Donna L.; Roberts, Beverly D.; Dezzutti, Charlene S.; Lal, Renu B.
In: Cancer Letters, Vol. 66, No. 1, 14.09.1992, p. 11-20.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization of a B-cell immunodominant epitope of human T-lymphotropic virus type 1 (HTLV-I) envelope gp46
AU - Lairmore, Michael Dale
AU - Rudolph, Donna L.
AU - Roberts, Beverly D.
AU - Dezzutti, Charlene S.
AU - Lal, Renu B.
PY - 1992/9/14
Y1 - 1992/9/14
N2 - The immune response elicited by a synthetic peptide derived from an immunodominant external envelope region (Env-5, amino acids 242-257) of human T-lymphotropic virus type 1 (HTLV-I) was tested in a rabbit model of HTLV-I infection. The synthetic peptide elicited a strong antibody response to the HTLV-I envelope protein gp46; however, these antibodies failed to inhibit HTLV-I-mediated cell fusion. Immunized rabbits were not protected from HTLV-I infection as determined by seroconversion to viral core proteins by immunoblot, HTLV-I p24 antigen detection in lymphocyte cultures and polymerase chain reaction for the HTLV-I provirus in lymphocyte DNA. Env-5 peptide immunization failed to induce T-cell lymphocyte proliferative responses in rabbits, but induced antibody responses in T-cell deficient Balb c nu/nu mice suggesting that the antigenic determinant represented by the Env-5 peptide is primarily a B-cell epitope. These results further define an immunodominant epitope of the HTLV-I envelope protein and suggest that potential synthetic peptide vaccines against HTLV-I infection must contain multiple antigens that induce both humoral and cellular immune reactivity.
AB - The immune response elicited by a synthetic peptide derived from an immunodominant external envelope region (Env-5, amino acids 242-257) of human T-lymphotropic virus type 1 (HTLV-I) was tested in a rabbit model of HTLV-I infection. The synthetic peptide elicited a strong antibody response to the HTLV-I envelope protein gp46; however, these antibodies failed to inhibit HTLV-I-mediated cell fusion. Immunized rabbits were not protected from HTLV-I infection as determined by seroconversion to viral core proteins by immunoblot, HTLV-I p24 antigen detection in lymphocyte cultures and polymerase chain reaction for the HTLV-I provirus in lymphocyte DNA. Env-5 peptide immunization failed to induce T-cell lymphocyte proliferative responses in rabbits, but induced antibody responses in T-cell deficient Balb c nu/nu mice suggesting that the antigenic determinant represented by the Env-5 peptide is primarily a B-cell epitope. These results further define an immunodominant epitope of the HTLV-I envelope protein and suggest that potential synthetic peptide vaccines against HTLV-I infection must contain multiple antigens that induce both humoral and cellular immune reactivity.
KW - animal model
KW - human
KW - human T-lymphotropic virus type 1
KW - synthetic peptide
KW - vaccine
UR - http://www.scopus.com/inward/record.url?scp=0026737339&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026737339&partnerID=8YFLogxK
U2 - 10.1016/0304-3835(92)90274-Y
DO - 10.1016/0304-3835(92)90274-Y
M3 - Article
C2 - 1360328
AN - SCOPUS:0026737339
VL - 66
SP - 11
EP - 20
JO - Cancer Letters
JF - Cancer Letters
SN - 0304-3835
IS - 1
ER -