Abstract
A Borrelia burgdorferi chromosomal gene encodes a 30-kDa antigen (P30) that has considerable homology with periplasmic substrate-binding proteins of Gram-negative bacteria, and is recognized by antibodies in sera from a subset of patients with Lyme disease and from B. burgdorferi-infected mice. The p30 gene is 801 nucleotides in length and P30 contains 267 amino acids, with a predicted molecular mass of 30 kDa. The P30 amino acid region 36-258 has homology to conserved domains of the oligopeptide permease A of Gram-negative bacteria. Immunofluorescence studies using murine anti-P30 serum suggest that P30 is on the outer surface of B. burgdorferi. P30 expression could be detected in representatives of all 3 subspecies of B. burgdorferi sensu lato, but not in all of the tested strains. Antibodies to P30 were detected in sera of 18 out of 82 patients (22%) with Lyme disease, including individuals with early- or late-stage infection. Although antibodies to P30 are present in the sera of C3H/HeN mice infected with B. burgdorferi for at least 90 days, immunization with recombinant P30 does not protect mice from infection. We conclude that P30 is a putative substrate-binding protein of B. burgdorferi and is immunologically recognized in human and murine Lyme borreliosis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 739-751 |
| Number of pages | 13 |
| Journal | Research in Microbiology |
| Volume | 147 |
| Issue number | 9 |
| DOIs | |
| State | Published - Nov 1996 |
| Externally published | Yes |
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Keywords
- Borrelia burgdorferi
- Lyme disease
- OppA homologue
- Outer surface protein
- p30
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Microbiology
Cite this
Characterization of a 30-kDa Borrelia burgdorferi substrate-binding protein homologue. / Das, S.; Shraga, D.; Cannon, C.; Lam, T. T.; Feng, S.; Brunet, L. R.; Telford, S. R.; Barthold, Stephen W; Flavell, R. A.; Fikrig, E.
In: Research in Microbiology, Vol. 147, No. 9, 11.1996, p. 739-751.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization of a 30-kDa Borrelia burgdorferi substrate-binding protein homologue
AU - Das, S.
AU - Shraga, D.
AU - Cannon, C.
AU - Lam, T. T.
AU - Feng, S.
AU - Brunet, L. R.
AU - Telford, S. R.
AU - Barthold, Stephen W
AU - Flavell, R. A.
AU - Fikrig, E.
PY - 1996/11
Y1 - 1996/11
N2 - A Borrelia burgdorferi chromosomal gene encodes a 30-kDa antigen (P30) that has considerable homology with periplasmic substrate-binding proteins of Gram-negative bacteria, and is recognized by antibodies in sera from a subset of patients with Lyme disease and from B. burgdorferi-infected mice. The p30 gene is 801 nucleotides in length and P30 contains 267 amino acids, with a predicted molecular mass of 30 kDa. The P30 amino acid region 36-258 has homology to conserved domains of the oligopeptide permease A of Gram-negative bacteria. Immunofluorescence studies using murine anti-P30 serum suggest that P30 is on the outer surface of B. burgdorferi. P30 expression could be detected in representatives of all 3 subspecies of B. burgdorferi sensu lato, but not in all of the tested strains. Antibodies to P30 were detected in sera of 18 out of 82 patients (22%) with Lyme disease, including individuals with early- or late-stage infection. Although antibodies to P30 are present in the sera of C3H/HeN mice infected with B. burgdorferi for at least 90 days, immunization with recombinant P30 does not protect mice from infection. We conclude that P30 is a putative substrate-binding protein of B. burgdorferi and is immunologically recognized in human and murine Lyme borreliosis.
AB - A Borrelia burgdorferi chromosomal gene encodes a 30-kDa antigen (P30) that has considerable homology with periplasmic substrate-binding proteins of Gram-negative bacteria, and is recognized by antibodies in sera from a subset of patients with Lyme disease and from B. burgdorferi-infected mice. The p30 gene is 801 nucleotides in length and P30 contains 267 amino acids, with a predicted molecular mass of 30 kDa. The P30 amino acid region 36-258 has homology to conserved domains of the oligopeptide permease A of Gram-negative bacteria. Immunofluorescence studies using murine anti-P30 serum suggest that P30 is on the outer surface of B. burgdorferi. P30 expression could be detected in representatives of all 3 subspecies of B. burgdorferi sensu lato, but not in all of the tested strains. Antibodies to P30 were detected in sera of 18 out of 82 patients (22%) with Lyme disease, including individuals with early- or late-stage infection. Although antibodies to P30 are present in the sera of C3H/HeN mice infected with B. burgdorferi for at least 90 days, immunization with recombinant P30 does not protect mice from infection. We conclude that P30 is a putative substrate-binding protein of B. burgdorferi and is immunologically recognized in human and murine Lyme borreliosis.
KW - Borrelia burgdorferi
KW - Lyme disease
KW - OppA homologue
KW - Outer surface protein
KW - p30
UR - http://www.scopus.com/inward/record.url?scp=0030298191&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030298191&partnerID=8YFLogxK
U2 - 10.1016/S0923-2508(97)85121-2
DO - 10.1016/S0923-2508(97)85121-2
M3 - Article
C2 - 9296108
AN - SCOPUS:0030298191
VL - 147
SP - 739
EP - 751
JO - Research in Microbiology
JF - Research in Microbiology
SN - 0923-2508
IS - 9
ER -