Characterization of a 30-kDa Borrelia burgdorferi substrate-binding protein homologue

S. Das, D. Shraga, C. Cannon, T. T. Lam, S. Feng, L. R. Brunet, S. R. Telford, Stephen W Barthold, R. A. Flavell, E. Fikrig

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A Borrelia burgdorferi chromosomal gene encodes a 30-kDa antigen (P30) that has considerable homology with periplasmic substrate-binding proteins of Gram-negative bacteria, and is recognized by antibodies in sera from a subset of patients with Lyme disease and from B. burgdorferi-infected mice. The p30 gene is 801 nucleotides in length and P30 contains 267 amino acids, with a predicted molecular mass of 30 kDa. The P30 amino acid region 36-258 has homology to conserved domains of the oligopeptide permease A of Gram-negative bacteria. Immunofluorescence studies using murine anti-P30 serum suggest that P30 is on the outer surface of B. burgdorferi. P30 expression could be detected in representatives of all 3 subspecies of B. burgdorferi sensu lato, but not in all of the tested strains. Antibodies to P30 were detected in sera of 18 out of 82 patients (22%) with Lyme disease, including individuals with early- or late-stage infection. Although antibodies to P30 are present in the sera of C3H/HeN mice infected with B. burgdorferi for at least 90 days, immunization with recombinant P30 does not protect mice from infection. We conclude that P30 is a putative substrate-binding protein of B. burgdorferi and is immunologically recognized in human and murine Lyme borreliosis.

Original languageEnglish (US)
Pages (from-to)739-751
Number of pages13
JournalResearch in Microbiology
Volume147
Issue number9
DOIs
StatePublished - Nov 1996
Externally publishedYes

Fingerprint

Borrelia burgdorferi
Lyme Disease
Carrier Proteins
Gram-Negative Bacteria
Serum
Antibodies
Periplasmic Binding Proteins
Borrelia burgdorferi Group
Amino Acids
Inbred C3H Mouse
Infection
Genes
Fluorescent Antibody Technique
Immunization
Nucleotides
Antigens

Keywords

  • Borrelia burgdorferi
  • Lyme disease
  • OppA homologue
  • Outer surface protein
  • p30

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Das, S., Shraga, D., Cannon, C., Lam, T. T., Feng, S., Brunet, L. R., ... Fikrig, E. (1996). Characterization of a 30-kDa Borrelia burgdorferi substrate-binding protein homologue. Research in Microbiology, 147(9), 739-751. https://doi.org/10.1016/S0923-2508(97)85121-2

Characterization of a 30-kDa Borrelia burgdorferi substrate-binding protein homologue. / Das, S.; Shraga, D.; Cannon, C.; Lam, T. T.; Feng, S.; Brunet, L. R.; Telford, S. R.; Barthold, Stephen W; Flavell, R. A.; Fikrig, E.

In: Research in Microbiology, Vol. 147, No. 9, 11.1996, p. 739-751.

Research output: Contribution to journalArticle

Das, S, Shraga, D, Cannon, C, Lam, TT, Feng, S, Brunet, LR, Telford, SR, Barthold, SW, Flavell, RA & Fikrig, E 1996, 'Characterization of a 30-kDa Borrelia burgdorferi substrate-binding protein homologue', Research in Microbiology, vol. 147, no. 9, pp. 739-751. https://doi.org/10.1016/S0923-2508(97)85121-2
Das, S. ; Shraga, D. ; Cannon, C. ; Lam, T. T. ; Feng, S. ; Brunet, L. R. ; Telford, S. R. ; Barthold, Stephen W ; Flavell, R. A. ; Fikrig, E. / Characterization of a 30-kDa Borrelia burgdorferi substrate-binding protein homologue. In: Research in Microbiology. 1996 ; Vol. 147, No. 9. pp. 739-751.
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