TY - JOUR
T1 - Characterization and immunomodulatory function comparison of various bursal-derived peptides isolated from the humoral central immune organ
AU - Feng, Xiuli
AU - Liu, Qing Tao
AU - Cao, Rui Bing
AU - Zhou, Bin
AU - Zhang, Yuan Peng
AU - Liu, Ke
AU - Liu, Xiao Dong
AU - Wei, Jian Chao
AU - Li, Xin Feng
AU - Chen, Pu Yan
PY - 2012/2/1
Y1 - 2012/2/1
N2 - The bursa of Fabricius (BF) is the acknowledged central immune organ, which is important to the B cell differentiation and antibody production. However, due to difficult purification, the immunomodulatory peptides from BF were little reported. In this study, the extract samples of BF were taken to a chromatographic analysis by RP-HPLC. Five novel low molecular weight peptides were isolated from BF, with amino acid sequences of YEYAY, RMYEE, GPPAT, AGCCNG, and RRL, and named as Bursal pentapeptide (BPP)-III, -IV, -V, and Bursal hexapeptide (BHP), and Bursal tripeptide (BTP), respectively. BSP-I, BSP-II, BPP-I and BPP-II are recently reported to be the bursal-derived bioactive peptides. In this paper, we analyzed the chemical formula and characteristics of these nine bursal-derived peptides. The immunization comparative experiment verified the different immunomodulatory activity of these nine bursal peptides on antibody and cytokine productions. Furthermore, the results showed that at reachable concentrations, BPP-II and BPP-I induced antibody productions, lymphocyte viabilities and cytokine responses in different dose-dependent manner in the immunized mice model, respectively. These results provided important orientations for the comprehensively understanding and study of the humoral central immune system of human, and provided a novel insight on the treatment of serious disease and immune improvement of human.
AB - The bursa of Fabricius (BF) is the acknowledged central immune organ, which is important to the B cell differentiation and antibody production. However, due to difficult purification, the immunomodulatory peptides from BF were little reported. In this study, the extract samples of BF were taken to a chromatographic analysis by RP-HPLC. Five novel low molecular weight peptides were isolated from BF, with amino acid sequences of YEYAY, RMYEE, GPPAT, AGCCNG, and RRL, and named as Bursal pentapeptide (BPP)-III, -IV, -V, and Bursal hexapeptide (BHP), and Bursal tripeptide (BTP), respectively. BSP-I, BSP-II, BPP-I and BPP-II are recently reported to be the bursal-derived bioactive peptides. In this paper, we analyzed the chemical formula and characteristics of these nine bursal-derived peptides. The immunization comparative experiment verified the different immunomodulatory activity of these nine bursal peptides on antibody and cytokine productions. Furthermore, the results showed that at reachable concentrations, BPP-II and BPP-I induced antibody productions, lymphocyte viabilities and cytokine responses in different dose-dependent manner in the immunized mice model, respectively. These results provided important orientations for the comprehensively understanding and study of the humoral central immune system of human, and provided a novel insight on the treatment of serious disease and immune improvement of human.
KW - Antibody production
KW - Bursal-derived peptides
KW - Cellular mediated immune response
KW - Immune model
KW - Reversed-phase HPLC
UR - http://www.scopus.com/inward/record.url?scp=84862800274&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84862800274&partnerID=8YFLogxK
U2 - 10.1016/j.peptides.2012.01.012
DO - 10.1016/j.peptides.2012.01.012
M3 - Article
C2 - 22286032
AN - SCOPUS:84862800274
VL - 33
SP - 258
EP - 264
JO - Peptides
JF - Peptides
SN - 0196-9781
IS - 2
ER -