Characterization and immunomodulatory function comparison of various bursal-derived peptides isolated from the humoral central immune organ

Xiuli Feng, Qing Tao Liu, Rui Bing Cao, Bin Zhou, Yuan Peng Zhang, Ke Liu, Xiao Dong Liu, Jian Chao Wei, Xin Feng Li, Pu Yan Chen

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

The bursa of Fabricius (BF) is the acknowledged central immune organ, which is important to the B cell differentiation and antibody production. However, due to difficult purification, the immunomodulatory peptides from BF were little reported. In this study, the extract samples of BF were taken to a chromatographic analysis by RP-HPLC. Five novel low molecular weight peptides were isolated from BF, with amino acid sequences of YEYAY, RMYEE, GPPAT, AGCCNG, and RRL, and named as Bursal pentapeptide (BPP)-III, -IV, -V, and Bursal hexapeptide (BHP), and Bursal tripeptide (BTP), respectively. BSP-I, BSP-II, BPP-I and BPP-II are recently reported to be the bursal-derived bioactive peptides. In this paper, we analyzed the chemical formula and characteristics of these nine bursal-derived peptides. The immunization comparative experiment verified the different immunomodulatory activity of these nine bursal peptides on antibody and cytokine productions. Furthermore, the results showed that at reachable concentrations, BPP-II and BPP-I induced antibody productions, lymphocyte viabilities and cytokine responses in different dose-dependent manner in the immunized mice model, respectively. These results provided important orientations for the comprehensively understanding and study of the humoral central immune system of human, and provided a novel insight on the treatment of serious disease and immune improvement of human.

Original languageEnglish (US)
Pages (from-to)258-264
Number of pages7
JournalPeptides
Volume33
Issue number2
DOIs
StatePublished - Feb 1 2012
Externally publishedYes

    Fingerprint

Keywords

  • Antibody production
  • Bursal-derived peptides
  • Cellular mediated immune response
  • Immune model
  • Reversed-phase HPLC

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

Cite this

Feng, X., Liu, Q. T., Cao, R. B., Zhou, B., Zhang, Y. P., Liu, K., Liu, X. D., Wei, J. C., Li, X. F., & Chen, P. Y. (2012). Characterization and immunomodulatory function comparison of various bursal-derived peptides isolated from the humoral central immune organ. Peptides, 33(2), 258-264. https://doi.org/10.1016/j.peptides.2012.01.012