Abstract
The Ca2+-phospholipid-dependent protein kinase, initially described by Takai et al. (J. Biol. Chem. 254, 3692-3695, 1979), has been identified in the anterior pituitary gland of the rat and sheep. The enzyme is essentially undetectable in initial cell extracts but marked activity is manifest following DEAE chromatography, suggesting the potential presence of an endogenous inhibitor of this enzyme. Two forms of this protein kinase exist in both sheep and rat anterior pituitary gland, both of which are similarly dependent upon Ca2+, phosphatidyl serine and diacylglycerol. Several endogenous substrates for this protein kinase have been observed in both the pars distalis and pars tuberalis of the sheep adenohypophysis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 107-112 |
| Number of pages | 6 |
| Journal | Molecular and Cellular Endocrinology |
| Volume | 34 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1984 |
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Keywords
- anterior pituitary gland
- Ca -phospholipid-dependent protein kinase
- protein phosphorylation
ASJC Scopus subject areas
- Endocrinology
- Endocrinology, Diabetes and Metabolism
Cite this
Characteristics of the adenohypophyseal Ca2+-phospholipid-dependent protein kinase. / Turgeon, Judith L; Ashcroft, S. J H; Waring, D. W.; Milewski, M. A.; Walsh, D. A.
In: Molecular and Cellular Endocrinology, Vol. 34, No. 2, 1984, p. 107-112.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characteristics of the adenohypophyseal Ca2+-phospholipid-dependent protein kinase
AU - Turgeon, Judith L
AU - Ashcroft, S. J H
AU - Waring, D. W.
AU - Milewski, M. A.
AU - Walsh, D. A.
PY - 1984
Y1 - 1984
N2 - The Ca2+-phospholipid-dependent protein kinase, initially described by Takai et al. (J. Biol. Chem. 254, 3692-3695, 1979), has been identified in the anterior pituitary gland of the rat and sheep. The enzyme is essentially undetectable in initial cell extracts but marked activity is manifest following DEAE chromatography, suggesting the potential presence of an endogenous inhibitor of this enzyme. Two forms of this protein kinase exist in both sheep and rat anterior pituitary gland, both of which are similarly dependent upon Ca2+, phosphatidyl serine and diacylglycerol. Several endogenous substrates for this protein kinase have been observed in both the pars distalis and pars tuberalis of the sheep adenohypophysis.
AB - The Ca2+-phospholipid-dependent protein kinase, initially described by Takai et al. (J. Biol. Chem. 254, 3692-3695, 1979), has been identified in the anterior pituitary gland of the rat and sheep. The enzyme is essentially undetectable in initial cell extracts but marked activity is manifest following DEAE chromatography, suggesting the potential presence of an endogenous inhibitor of this enzyme. Two forms of this protein kinase exist in both sheep and rat anterior pituitary gland, both of which are similarly dependent upon Ca2+, phosphatidyl serine and diacylglycerol. Several endogenous substrates for this protein kinase have been observed in both the pars distalis and pars tuberalis of the sheep adenohypophysis.
KW - anterior pituitary gland
KW - Ca -phospholipid-dependent protein kinase
KW - protein phosphorylation
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UR - http://www.scopus.com/inward/citedby.url?scp=0021289244&partnerID=8YFLogxK
U2 - 10.1016/0303-7207(84)90061-3
DO - 10.1016/0303-7207(84)90061-3
M3 - Article
C2 - 6232162
AN - SCOPUS:0021289244
VL - 34
SP - 107
EP - 112
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
SN - 0303-7207
IS - 2
ER -