Characteristics of a chimeric enzyme engineered from two rice α-amylase isozymes

M. Terashima, M. Kawai, M. H. Kumagai, R. L. Rodriguez, S. Katoh

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

A chimeric enzyme, engineered from two rice α-amylase isozymes, Amy1A and Amy3D, showed unique characteristics in soluble-starch and maltoheptaose hydrolysis. Effects of pH on soluble-starch hydrolysis and the thermostability of the chimeric enzyme were similar to those of the isozyme Amy3D. The previous study revealed that Amy1A shows high activity in soluble-starch hydrolysis and low activity in oligosaccharide degradation, while Amy3D shows low activity in soluble-starch hydrolysis and high activity in oligosaccharide degradation. The chimeric enzyme showed high activities in both soluble-starch hydrolysis and oligosaccharide degradation. These results suggest that protein modules of highly homologous enzymes are interchangeable, and that a novel enzyme with unique characteristics can be obtained by creating a chimeric enzyme.

Original languageEnglish (US)
Pages (from-to)607-611
Number of pages5
JournalApplied Microbiology and Biotechnology
Volume45
Issue number5
DOIs
StatePublished - 1996

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Microbiology

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