Characteristics of a chimeric enzyme engineered from two rice α-amylase isozymes

M. Terashima, M. Kawai, M. H. Kumagai, R. L. Rodriguez, S. Katoh

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A chimeric enzyme, engineered from two rice α-amylase isozymes, Amy1A and Amy3D, showed unique characteristics in soluble-starch and maltoheptaose hydrolysis. Effects of pH on soluble-starch hydrolysis and the thermostability of the chimeric enzyme were similar to those of the isozyme Amy3D. The previous study revealed that Amy1A shows high activity in soluble-starch hydrolysis and low activity in oligosaccharide degradation, while Amy3D shows low activity in soluble-starch hydrolysis and high activity in oligosaccharide degradation. The chimeric enzyme showed high activities in both soluble-starch hydrolysis and oligosaccharide degradation. These results suggest that protein modules of highly homologous enzymes are interchangeable, and that a novel enzyme with unique characteristics can be obtained by creating a chimeric enzyme.

Original languageEnglish (US)
Pages (from-to)607-611
Number of pages5
JournalApplied Microbiology and Biotechnology
Volume45
Issue number5
DOIs
StatePublished - 1996

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Amylases
Isoenzymes
Starch
Enzymes
Hydrolysis
Oligosaccharides
Degradation
Oryza
Thermodynamic properties
Proteins

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Microbiology

Cite this

Characteristics of a chimeric enzyme engineered from two rice α-amylase isozymes. / Terashima, M.; Kawai, M.; Kumagai, M. H.; Rodriguez, R. L.; Katoh, S.

In: Applied Microbiology and Biotechnology, Vol. 45, No. 5, 1996, p. 607-611.

Research output: Contribution to journalArticle

Terashima, M. ; Kawai, M. ; Kumagai, M. H. ; Rodriguez, R. L. ; Katoh, S. / Characteristics of a chimeric enzyme engineered from two rice α-amylase isozymes. In: Applied Microbiology and Biotechnology. 1996 ; Vol. 45, No. 5. pp. 607-611.
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