Abstract
We report the analysis of the vibrational modes of intranuclear protein inclusions isolated from the brain of human subjects with the Fragile X-associated tremor/ataxia syndrome (FXTAS). In this preliminary study, Raman spectra of optically trapped inclusions were measured and analysed to determine protein composition and structure. Our main findings are as follows:(1) ThespectraofproteininclusionsarecharacteristicofH2AandH2Bhistones, whichcorrelatewithpreviousmassspectrometry (MS) studies; (2) Tyrosine is present in its OH form and exposed at the protein surface; (3) Zn and to a lesser extent Cu bound to histidine side chains are detected in the inclusions; (4) The tryptophan side-chain torsion angle is calculated to be 102° (5) Several potential spectroscopic markers for the inclusions of FXTAS are identified. Theseresults show the capability of using laser tweezers Raman spectroscopy to identify protein inclusions in a non-perturbative way and to gain further insight into the pathogenesis and progression of FXTAS in human subjects and in experimental models of this disorder.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 33-39 |
| Number of pages | 7 |
| Journal | Journal of Raman Spectroscopy |
| Volume | 41 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 2010 |
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Keywords
- Fragilex-associated tremor/ataxiasyndrome
- Intranuclearinclusions
- Lasertweezers
- Proteininclusions
- Ramanspectroscopy
ASJC Scopus subject areas
- Spectroscopy
- Materials Science(all)
Cite this
Characterisation of FXTAS related isolated intranuclear protein inclusions using laser tweezers raman spectroscopy. / Moritz, Tobias J.; Brunberg, James A; Krol, Denise M.; Wachsmann-Hogiu, Sebastian; Lane, Stephen M.; Chan, James W.
In: Journal of Raman Spectroscopy, Vol. 41, No. 1, 01.2010, p. 33-39.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterisation of FXTAS related isolated intranuclear protein inclusions using laser tweezers raman spectroscopy
AU - Moritz, Tobias J.
AU - Brunberg, James A
AU - Krol, Denise M.
AU - Wachsmann-Hogiu, Sebastian
AU - Lane, Stephen M.
AU - Chan, James W
PY - 2010/1
Y1 - 2010/1
N2 - We report the analysis of the vibrational modes of intranuclear protein inclusions isolated from the brain of human subjects with the Fragile X-associated tremor/ataxia syndrome (FXTAS). In this preliminary study, Raman spectra of optically trapped inclusions were measured and analysed to determine protein composition and structure. Our main findings are as follows:(1) ThespectraofproteininclusionsarecharacteristicofH2AandH2Bhistones, whichcorrelatewithpreviousmassspectrometry (MS) studies; (2) Tyrosine is present in its OH form and exposed at the protein surface; (3) Zn and to a lesser extent Cu bound to histidine side chains are detected in the inclusions; (4) The tryptophan side-chain torsion angle is calculated to be 102° (5) Several potential spectroscopic markers for the inclusions of FXTAS are identified. Theseresults show the capability of using laser tweezers Raman spectroscopy to identify protein inclusions in a non-perturbative way and to gain further insight into the pathogenesis and progression of FXTAS in human subjects and in experimental models of this disorder.
AB - We report the analysis of the vibrational modes of intranuclear protein inclusions isolated from the brain of human subjects with the Fragile X-associated tremor/ataxia syndrome (FXTAS). In this preliminary study, Raman spectra of optically trapped inclusions were measured and analysed to determine protein composition and structure. Our main findings are as follows:(1) ThespectraofproteininclusionsarecharacteristicofH2AandH2Bhistones, whichcorrelatewithpreviousmassspectrometry (MS) studies; (2) Tyrosine is present in its OH form and exposed at the protein surface; (3) Zn and to a lesser extent Cu bound to histidine side chains are detected in the inclusions; (4) The tryptophan side-chain torsion angle is calculated to be 102° (5) Several potential spectroscopic markers for the inclusions of FXTAS are identified. Theseresults show the capability of using laser tweezers Raman spectroscopy to identify protein inclusions in a non-perturbative way and to gain further insight into the pathogenesis and progression of FXTAS in human subjects and in experimental models of this disorder.
KW - Fragilex-associated tremor/ataxiasyndrome
KW - Intranuclearinclusions
KW - Lasertweezers
KW - Proteininclusions
KW - Ramanspectroscopy
UR - http://www.scopus.com/inward/record.url?scp=74349085329&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=74349085329&partnerID=8YFLogxK
U2 - 10.1002/jrs.2436
DO - 10.1002/jrs.2436
M3 - Article
AN - SCOPUS:74349085329
VL - 41
SP - 33
EP - 39
JO - Journal of Raman Spectroscopy
JF - Journal of Raman Spectroscopy
SN - 0377-0486
IS - 1
ER -