Changes of P2X-purinoceptors in rat vas deferens and spleen caused by long term guanethidine treatment

Min Zhao, P. Milner

Research output: Contribution to journalArticle

Abstract

Using [3H] α, β-Methylene ATP ([3H] α, β-MeATP) as the radioligand, changes in the P2X-purinoceptor density and affinity in rat vas deferens and spleen have been examined following long-term guanethidine treatment. Large doses of guanethidine (50mg/kg) were given daily for 3 weeks to 8-day-old rat pups, which were killed at 14 weeks of age. The results showed that in guanethidine-treated rats P2X-purinoceptor densities (Bmax) of both the high and low affinity components were significantly increased (P<0.05). The Bmax for the high affinity site were increased significantly (P<0.05): from 1.0±0.2 to 3.4±0.8 (pmol/mg.protein) in the spleen, from 3.2±0.7 to 22.3±4.3 in the vas deferens, while the affinity was decreased as shown by Kd from 5.4±1.0 to 18.1±2.5 nM in the spleen and from 1.2±0.3 to 5.4±2.2 nM in the vas deferens (P<0.01) after guanethidine treatment. The Bmax for the low affinity site were also increased significantly (P<0.05) from 16.0±3.6 to 160.0±10.6 (pmol/mg. protein) in the spleen, from 9.7±2.0 to 296.4±52.5 in the vas deferens, while the affinity was decreased as shown by Kd from 222±57 to 882±298 nM (P=0.08) in the spleen and from 11.3±4.2 to 122.7±24.2 nM in the vas deferens (P<0.05) after guanethidine treatment. It is concluded that following sympathectomy and loss of the cotransmitter ATP in the vas deferens and spleen, there is an increase in density of P2X receptor sites, although a decrease in receptor affinity.

Original languageEnglish (US)
Pages (from-to)23-29
Number of pages7
JournalBiomedical Research
Volume14
Issue number1
StatePublished - Jan 2003
Externally publishedYes

Fingerprint

Purinergic P2X Receptors
Guanethidine
Vas Deferens
Rats
Spleen
Adenosine Triphosphate
Sympathectomy
Proteins

Keywords

  • [H] α, β-Methylene ATP
  • Guanethidine treatment
  • P2X-purinoceptor
  • Purinergic neurotransmission

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Changes of P2X-purinoceptors in rat vas deferens and spleen caused by long term guanethidine treatment. / Zhao, Min; Milner, P.

In: Biomedical Research, Vol. 14, No. 1, 01.2003, p. 23-29.

Research output: Contribution to journalArticle

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abstract = "Using [3H] α, β-Methylene ATP ([3H] α, β-MeATP) as the radioligand, changes in the P2X-purinoceptor density and affinity in rat vas deferens and spleen have been examined following long-term guanethidine treatment. Large doses of guanethidine (50mg/kg) were given daily for 3 weeks to 8-day-old rat pups, which were killed at 14 weeks of age. The results showed that in guanethidine-treated rats P2X-purinoceptor densities (Bmax) of both the high and low affinity components were significantly increased (P<0.05). The Bmax for the high affinity site were increased significantly (P<0.05): from 1.0±0.2 to 3.4±0.8 (pmol/mg.protein) in the spleen, from 3.2±0.7 to 22.3±4.3 in the vas deferens, while the affinity was decreased as shown by Kd from 5.4±1.0 to 18.1±2.5 nM in the spleen and from 1.2±0.3 to 5.4±2.2 nM in the vas deferens (P<0.01) after guanethidine treatment. The Bmax for the low affinity site were also increased significantly (P<0.05) from 16.0±3.6 to 160.0±10.6 (pmol/mg. protein) in the spleen, from 9.7±2.0 to 296.4±52.5 in the vas deferens, while the affinity was decreased as shown by Kd from 222±57 to 882±298 nM (P=0.08) in the spleen and from 11.3±4.2 to 122.7±24.2 nM in the vas deferens (P<0.05) after guanethidine treatment. It is concluded that following sympathectomy and loss of the cotransmitter ATP in the vas deferens and spleen, there is an increase in density of P2X receptor sites, although a decrease in receptor affinity.",
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N2 - Using [3H] α, β-Methylene ATP ([3H] α, β-MeATP) as the radioligand, changes in the P2X-purinoceptor density and affinity in rat vas deferens and spleen have been examined following long-term guanethidine treatment. Large doses of guanethidine (50mg/kg) were given daily for 3 weeks to 8-day-old rat pups, which were killed at 14 weeks of age. The results showed that in guanethidine-treated rats P2X-purinoceptor densities (Bmax) of both the high and low affinity components were significantly increased (P<0.05). The Bmax for the high affinity site were increased significantly (P<0.05): from 1.0±0.2 to 3.4±0.8 (pmol/mg.protein) in the spleen, from 3.2±0.7 to 22.3±4.3 in the vas deferens, while the affinity was decreased as shown by Kd from 5.4±1.0 to 18.1±2.5 nM in the spleen and from 1.2±0.3 to 5.4±2.2 nM in the vas deferens (P<0.01) after guanethidine treatment. The Bmax for the low affinity site were also increased significantly (P<0.05) from 16.0±3.6 to 160.0±10.6 (pmol/mg. protein) in the spleen, from 9.7±2.0 to 296.4±52.5 in the vas deferens, while the affinity was decreased as shown by Kd from 222±57 to 882±298 nM (P=0.08) in the spleen and from 11.3±4.2 to 122.7±24.2 nM in the vas deferens (P<0.05) after guanethidine treatment. It is concluded that following sympathectomy and loss of the cotransmitter ATP in the vas deferens and spleen, there is an increase in density of P2X receptor sites, although a decrease in receptor affinity.

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