Changes in thymidine kinase activity during differentiation of HL-60 leukemic cells

Thomas J. Borelli, Suzanne Miyamoto, Stanley J. Wertheimer, Joseph M. Wu

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Induction of granulocyte maturation in HL-60 leukemic cells by DMSO (1.2%) or RA (1 μM) is accompanied by a 50-60% decrease in cellular thymidine kinase activity. Similarly, the differentiation of HL-60 cells into monocyte-macrophage phenotype by the addition of PMA is paralleled by a 60-80% suppression of thymidine kinase specific activity. Measurement of thymidine kinase kinetic parameters shows that the Vmax decreases from 0.7 pmol/min in control cells to 0.43 pmol/min in PMA-treated cells and to 0.38 pmol/min in RA-treated cells. The Km of the enzyme is not affected by either inducing agent and remains at 2.1 μM. Studies with PMA analogs suggest that thymidine kinase modulation is coupled to HL-60 differentiation.

Original languageEnglish (US)
Pages (from-to)469-473
Number of pages5
JournalLeukemia Research
Volume11
Issue number5
DOIs
StatePublished - 1987
Externally publishedYes

Keywords

  • differentiation
  • leukemia
  • Thymidine kinase

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

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