Changes in the carbohydrate content of airway epithelium induced by human neutrophil elastase in the hamster

V. K. Dimitriadis, T. G. Christensen, E. C. Lucey, G. L. Snider, Charles Plopper

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Hamster airway epithelial secretory cells were investigated by light and electron microscopic cytochemistry to study possible changes in their carbohydrate content induced by human neutrophil elastase (HNE), an agent known to cause replacement of Clara cells by mucous cells in hamster bronchi. Characterization of secretory cell carbohydrates by the AB/PAS, PA-TCH-SP, HID-TCH-SP, and UD-TCH-SP sequences indicated the existence of periodate-reactive acidic glycoconjugates, but the absence of sulfated or carboxylated glycoconjugates in both treated and control animals. Differences were seen in the quality and quantity of historeactive carbohydrates throughout various regions in the lower respiratory tract. This was especially evident in the HNE-treated animals. It is conclued that the HNE-induced expression of the mucous cell phenotype is associated with an increase in the amount of neutral and acidic nonsulfated and noncarboxylated polysaccharides stored in the secretory granules of these cells.

Original languageEnglish (US)
Pages (from-to)731-742
Number of pages12
JournalExperimental Lung Research
Volume18
Issue number5
DOIs
StatePublished - Jan 1 1992

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Leukocyte Elastase
Cricetinae
Glycoconjugates
Epithelium
Carbohydrates
Animals
Polysaccharides
Histocytochemistry
Cells
Secretory Vesicles
Bronchi
Electrons
Respiratory System
Epithelial Cells
Phenotype
Light

ASJC Scopus subject areas

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry

Cite this

Changes in the carbohydrate content of airway epithelium induced by human neutrophil elastase in the hamster. / Dimitriadis, V. K.; Christensen, T. G.; Lucey, E. C.; Snider, G. L.; Plopper, Charles.

In: Experimental Lung Research, Vol. 18, No. 5, 01.01.1992, p. 731-742.

Research output: Contribution to journalArticle

Dimitriadis, V. K. ; Christensen, T. G. ; Lucey, E. C. ; Snider, G. L. ; Plopper, Charles. / Changes in the carbohydrate content of airway epithelium induced by human neutrophil elastase in the hamster. In: Experimental Lung Research. 1992 ; Vol. 18, No. 5. pp. 731-742.
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